Gliemroth Jan, Zulewski Henryk, Arnold Hans, Terzis A Jorge A
Department of Neurosurgery, Medical University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.
Neurosurg Rev. 2003 May;26(2):117-24. doi: 10.1007/s10143-003-0258-9.
This study describes the migration, proliferation, and invasion behaviour of two human glioma cell lines, GaMg and U-87 Mg, grown as multicellular tumour spheroids after 3-hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase inhibitor (simvastatin) therapy. Migration and proliferation studies were performed using simvastatin in concentrations of 0.2-30 microg/ml(-1). A coculture system in which tumour spheroids were confronted with foetal rat brain aggregates was used for invasion studies. A dose-dependent growth and migratory inhibitory response to simvastatin treatment was observed. Marked invasion of the glioma spheroids into the brain aggregates could be seen in both treated and nontreated groups. Simvastatin therapy inhibits tumour cell growth and migration, but the invasiveness of the remaining tumour cells seems to be unaffected.
本研究描述了两种人类胶质瘤细胞系GaMg和U - 87 Mg在3 - 羟基 - 3 - 甲基戊二酰辅酶A（HMGCoA）还原酶抑制剂（辛伐他汀）治疗后形成多细胞肿瘤球体时的迁移、增殖和侵袭行为。使用浓度为0.2 - 30微克/毫升（-1）的辛伐他汀进行迁移和增殖研究。采用肿瘤球体与胎鼠脑聚集体相互作用的共培养系统进行侵袭研究。观察到对辛伐他汀治疗有剂量依赖性的生长和迁移抑制反应。在治疗组和未治疗组中均可见胶质瘤球体对脑聚集体的明显侵袭。辛伐他汀治疗可抑制肿瘤细胞生长和迁移，但剩余肿瘤细胞的侵袭性似乎未受影响。
