da Costa Nuno, McGillivray Christine, Chang Kin-Chow
Veterinary Molecular Medicine Laboratory, Institute of Comparative Medicine, University of Glasgow Veterinary School, Glasgow, Scotland.
Anat Rec A Discov Mol Cell Evol Biol. 2003 Aug;273(2):731-40. doi: 10.1002/ar.a.10083.
Our knowledge of the temporal expression of postnatal (adult) fast myosin heavy chain (MyHC) isoforms (2a, 2x, and 2b) in prenatal muscles is limited. Using the pig as a target species and large-animal model, we report on the qualitative and quantitative expression of the major post- and prenatal MyHC isoforms during gestation, as determined by TaqMan real-time PCR and immunohistochemistry. We found that postnatal fast MyHC mRNAs and proteins were expressed much earlier in the pig (gestation day 35) than was previously reported in small mammals. There was a high degree of coexpression and colocalisation of pre- and postnatal MyHC mRNAs and proteins in prenatal muscles. During a period of prenatal muscle growth (gestation days 35-77), relative expression of MyHC isoforms (embryonic > 2a > 2x > 2b) correlated with the gene order in the skeletal MyHC cluster, which suggests the possible presence of cis-acting elements on the same side as the MyHC embryonic gene associated with temporal regulation.
我们对出生后（成体）快速肌球蛋白重链（MyHC）亚型（2a、2x和2b）在产前肌肉中的时间表达的了解有限。以猪作为目标物种和大型动物模型，我们报告了通过TaqMan实时PCR和免疫组织化学测定的妊娠期间主要产后和产前MyHC亚型的定性和定量表达。我们发现，出生后快速MyHC mRNA和蛋白质在猪中（妊娠第35天）的表达比先前在小型哺乳动物中的报道要早得多。产前肌肉中产前和产后MyHC mRNA及蛋白质存在高度的共表达和共定位。在产前肌肉生长阶段（妊娠第35 - 77天），MyHC亚型的相对表达（胚胎型>2a>2x>2b）与骨骼肌MyHC基因簇中的基因顺序相关，这表明与时间调控相关的顺式作用元件可能存在于与MyHC胚胎基因同侧。
