Hamamoto Kouko, Mizuno Yasuharu, Koike Ryoji, Yamaoka Ryozo, Takahashi Toshio, Takahashi Yoshiyuki
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries: 1-15-1, Tokura, Kokubunji, Tokyo 185-8511, Japan.
Shokuhin Eiseigaku Zasshi. 2003 Apr;44(2):114-8. doi: 10.3358/shokueishi.44.114.
A reversed-phase HPLC method with ultraviolet detection using p-nitrophenyl hydrazine as a pre-column derivatizing reagent was investigated for the determination of the antibiotic spectinomycin (SPCM) in muscle, liver, kidney and fat of chicken and swine. SPCM was extracted from samples with 10% trichloroacetic acid saturated with EDTA-2Na, and then cleaned up with coupled Sep-Pak Plus PS-2 cartridges. The detection limit of SPCM was 0.02 microgram (potency)/g. Recoveries of SPCM ranged from 77.4 to 97.4% for chicken tissues and from 74.5 to 91.8% for swine tissues. The present method was used for the analysis of chicken tissues after the 11th day of withdrawal (SPCM-medicated drinking water: 500 mg (potency)/L, for 7 days), and swine tissues after the 14th day of withdrawal (SPCM-medicated feed: 100 mg (potency)/kg, for 7 days). Results showed that SPCM concentrations were lower than the MRLs in all tissues.
研究了一种以对硝基苯肼为柱前衍生试剂、紫外检测的反相高效液相色谱法，用于测定鸡肉和猪肉的肌肉、肝脏、肾脏和脂肪中的抗生素壮观霉素（SPCM）。用10%的三氯乙酸（用乙二胺四乙酸二钠饱和）从样品中提取SPCM，然后用串联的Sep-Pak Plus PS-2柱进行净化。SPCM的检测限为0.02微克（效价）/克。鸡肉组织中SPCM的回收率为77.4%至97.4%，猪肉组织中为74.5%至91.8%。本方法用于分析停药第11天（SPCM药物饮用水：500毫克（效价）/升，持续7天）后的鸡肉组织，以及停药第14天（SPCM药物饲料：100毫克（效价）/千克，持续7天）后的猪肉组织。结果表明，所有组织中的SPCM浓度均低于最高残留限量。
