组胺缺乏（组氨酸脱羧酶基因敲除）小鼠繁殖能力受损主要是由雄性交配行为减少所致。

Impaired reproduction of histamine deficient (histidine-decarboxylase knockout) mice is caused predominantly by a decreased male mating behavior.

作者信息

Pár Gabriella, Szekeres-Barthó Júlia, Buzás Edit, Pap Erna, Falus András

机构信息

Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pecs, Pecs, Hungary.

出版信息

Am J Reprod Immunol. 2003 Aug;50(2):152-8. doi: 10.1034/j.1600-0897.2003.00066.x.

DOI:10.1034/j.1600-0897.2003.00066.x

PMID:12846679

Abstract

PROBLEM

Histamine induces a Th2 shift. As successful allopregnancy is characterized by a peripheral Th2 dominance, we investigated the role of histamine in reproduction.

METHOD OF STUDY

HDC knockout (HDC-/-) or wild-type (HDC+/+) mice kept on histamine-deficient or normal diet were mated. Appearance of vaginal plugs indicated day 0.5 of pregnancy. On day 10.5 uteri were inspected. Splenic IFN-gamma production and cytotoxic activity were determined.

RESULTS

In HDC+/+ or HDC-/- females on normal diet, plugs appeared between 3 and 6 days. In 80% of the (HDC-/-)/(HDC-/-) matings on histamine-deficient diet, no vaginal plugs were observed for more than 1 month. After replacing males with the wild type, plugs appeared within 3 days. In HDC-/- mice, litter size was lower than in HDC+/+ animals. Cytotoxicity and IFN-gamma production were significantly increased in non-pregnant histamine-deficient mice, but not in pregnant mice.

CONCLUSION

Histamine affects male mating behavior, but is not indispensable for successful pregnancy.

摘要

问题

组胺会诱导Th2偏移。由于成功的同种异体妊娠的特征是外周Th2占优势，我们研究了组胺在生殖中的作用。

研究方法

将饲养在组胺缺乏或正常饮食条件下的HDC基因敲除（HDC-/-）或野生型（HDC+/+）小鼠进行交配。阴道栓的出现表明妊娠第0.5天。在第10.5天检查子宫。测定脾脏干扰素-γ的产生和细胞毒性活性。

结果

在正常饮食的HDC+/+或HDC-/-雌性小鼠中，阴道栓在3至6天出现。在组胺缺乏饮食条件下80%的（HDC-/-）/（HDC-/-）交配中，超过1个月未观察到阴道栓。用野生型雄性小鼠替换后，3天内出现阴道栓。在HDC-/-小鼠中，产仔数低于HDC+/+动物。非妊娠组胺缺乏小鼠的细胞毒性和干扰素-γ产生显著增加，但妊娠小鼠未出现这种情况。