Kretschmer V, Tesche U, Richter P
Department of Transfusion Medicine and Coagulation Physiology, University Clinics, Marburg, FRG.
Beitr Infusionsther. 1992;30:112-21.
By use of the new top-and-bottom bag system and automatic separators the quality of red cell concentrates (RCC) and fresh frozen plasma (FFP) could be markedly improved. Simultaneous preparation of storable single-donor platelet concentrates (PC) resulted, however, in too high losses of red cells and plasma. Through modification of the pressure plate of the separators and variation of the residual buffy coat (BC) volumes we tried to optimize the separation in this respect. In a second study we tried to establish the preparation of platelet-rich plasma (PRP) using top-and-bottom bag systems and automatic separators.
1st study: 149 whole-blood units (Biopack U, quadruple systems) were separated into components either with Optipress (Opt, n = 58) or with Biotrans Separator (Bio, n = 91). To Optipress additional plates, 3-6 mm thick, were attached and the residual BC volume was varied by different adjustments of the distance screw. The Biotrans Separator was used with a plane pressure plate as well as with a modified plate having a transverse groove; the residual BC volume was adjusted to 50-120 ml. 2nd study: 15 whole-blood units were separated into PRP and RCC either using the Optipress (standard version, n = 5) or the Biotrans Separator (alternating opening of clamps, standard plate, n = 10).
1st study: Both separations could be improved. The loss of red cells was significantly lower in case of the Biotrans Separator: Bio 15.6%, Opt 25.9%, p < 0.001. Other separation parameters showed no relevant difference between both separators. 2nd study: By use of the Biotrans Separator significantly less platelets could be separated (Bio 75.1 x 10(9), Opt 87.0 x 10(9), p < 0.05), but the PRP clearly was contaminated with less leukocytes (Bio 51.0 x 10(6), Opt 458.9 x 10(6), p < 0.01). The contamination of the RCC with leukocytes (about 20%) was markedly improved in both methods when compared with conventional preparations. The mean loss of red cells was 12.8% in both methods.
通过使用新型上下袋系统和自动分离器，红细胞浓缩物（RCC）和新鲜冰冻血浆（FFP）的质量可得到显著提高。然而，同时制备可储存的单供体血小板浓缩物（PC）会导致红细胞和血浆的损失过高。我们试图通过改变分离器的压板以及残余白膜（BC）体积来优化这方面的分离效果。在第二项研究中，我们试图利用上下袋系统和自动分离器来制备富含血小板血浆（PRP）。
第一项研究：149个全血单位（Biopack U，四联系统）使用Optipress（Opt，n = 58）或Biotrans分离器（Bio，n = 91）分离成各成分。在Optipress上附加了3 - 6毫米厚的额外压板，并通过距离螺丝的不同调整来改变残余BC体积。Biotrans分离器使用平面压板以及带有横向凹槽的改良压板；残余BC体积调整为50 - 120毫升。第二项研究：15个全血单位使用Optipress（标准版本，n = 5）或Biotrans分离器（夹钳交替打开，标准板，n = 10）分离成PRP和RCC。
第一项研究：两种分离方法均得到改进。使用Biotrans分离器时红细胞损失显著更低：Bio为15.6%，Opt为25.9%，p < 0.001。其他分离参数在两种分离器之间未显示出显著差异。第二项研究：使用Biotrans分离器时可分离出的血小板显著更少（Bio为75.1×10⁹，Opt为87.0×10⁹，p < 0.05），但PRP中白细胞污染明显更少（Bio为51.0×10⁶，Opt为458.9×10⁶，p < 0.01）。与传统制备方法相比，两种方法中RCC的白细胞污染（约20%）均得到显著改善。两种方法中红细胞的平均损失均为12.8%。
