Holzberger G, Frank R, Boehm B O, Seidl S
Institut für Immunhämatologie und BSD Hessen, BRD.
Beitr Infusionsther. 1992;30:341-3.
Genetic polymorphism in the HLA class II region has been identified by the analysis of the polymerase chain reaction (PCR) products using sequence-specific oligonucleotide (SSO). The PCR-SSO method permits precise and direct analysis of allelic variations with as little as 1 microgram of genomic DNA. The standardized, uniform hybridization and critical wash protocol of the Eurotransplant typing kit enables HLA typing independent of gene expression and quality of lymphocytes. One of the advantages of this technique is that the definition of splits is much better than typing by serology.
通过使用序列特异性寡核苷酸（SSO）分析聚合酶链反应（PCR）产物，已确定了HLA II类区域的基因多态性。PCR-SSO方法允许使用低至1微克的基因组DNA精确直接地分析等位基因变异。Eurotransplant分型试剂盒标准化、统一的杂交和严格的洗涤方案使HLA分型独立于淋巴细胞的基因表达和质量。该技术的优点之一是对裂分的定义比血清学分型要好得多。
