Schakenraad J M, van der Mei H C, Rouxhet P G, Busscher H J
Center for Biomedical Technology, University of Groningen, The Netherlands.
Cell Biophys. 1992 Feb;20(1):57-67. doi: 10.1007/BF02782654.
Elemental surface concentration ratios N/C,O/C, and P/C of fibroblasts, HELA epithelial cells, and smooth muscle cells, prior to and after washing in the absence or presence of serum proteins, were determined by X-ray photoelectron spectroscopy. Cell surfaces appeared to adsorb hardly any serum proteins, and the relatively high P/C, as compared to N/C and O/C, elemental surface concentration ratio indicated that the cell surfaces consisted mainly of the phospholipid bilayer, with little or no proteins present. The lack of adsorption of serum proteins to the cell surfaces seems at odds with the common notion that cells require adhesive proteins in order to adhere and spread. However, the adsorption behavior of cellularly produced proteins may be completely different, particularly since they seem to be able to displace adsorbed serum proteins from biomaterials surfaces. Interestingly, only HELA epithelial cells (a tumor cell line) appeared to adsorb a very small amount of proteins.
通过X射线光电子能谱测定了成纤维细胞、HELA上皮细胞和平滑肌细胞在有无血清蛋白存在的情况下洗涤前后的元素表面浓度比N/C、O/C和P/C。细胞表面似乎几乎不吸附任何血清蛋白，与N/C和O/C相比，相对较高的P/C元素表面浓度比表明细胞表面主要由磷脂双层组成，几乎没有或不存在蛋白质。血清蛋白在细胞表面缺乏吸附似乎与细胞需要粘附蛋白才能粘附和铺展的普遍观念不一致。然而，细胞产生的蛋白质的吸附行为可能完全不同，特别是因为它们似乎能够从生物材料表面取代吸附的血清蛋白。有趣的是，只有HELA上皮细胞（一种肿瘤细胞系）似乎吸附了极少量的蛋白质。
