Van Nassauw L, Harrisson F, Callebaut M
University Centre of Antwerp, Institute of Human Anatomy and Embryology, Belgium.
Eur J Morphol. 1992;30(4):275-88.
We localized alpha-smooth muscle actin (alpha-SMA) in the quail ovary, using the peroxidase-anti-peroxidase technique. Special attention was paid to the influence of fixation on the immunoreactivity of the antigen. The immunostaining of alpha-SMA largely depended on the nature of the fixative. The antigen could most successfully be localized in ovaries fixed in Carnoy's fluid. We also localized alpha-SMA and desmin in semi-thin glycol methacrylate sections of the pre-ovulatory follicle, using the immunogold-silver staining method. The sections were pretreated with Lugol's iodine or sodium metaperiodate to enhance the immunoreactivity. alpha-SMA was demonstrated in the cells of the chordae, the tunica albuginea, and the theca externa of each follicle. These structures were inter-connected, forming an ovarian suspensory apparatus. The thecal cells of prelampbrush follicles also expressed alpha-SMA. In the wall of the pre-ovulatory follicle, desmin was found in the cells of the chordae and the tunica albuginea, and in a few cells of the theca externa. In the theca interna, desmin, and sometimes alpha-SMA, was observed in cells adjacent to the endothelium of sinusoids, which are probably pericytes. Our results support the hypothesis that in birds the ovarian follicles possess a thecal contractile system, that is presumably involved in the ovulatory process.
我们采用过氧化物酶-抗过氧化物酶技术，对鹌鹑卵巢中的α-平滑肌肌动蛋白（α-SMA）进行定位。特别关注了固定对抗原免疫反应性的影响。α-SMA的免疫染色很大程度上取决于固定剂的性质。抗原最成功地定位于用卡诺氏液固定的卵巢中。我们还采用免疫金银染色法，在排卵前卵泡的半薄甲基丙烯酸乙二醇酯切片中对α-SMA和结蛋白进行定位。切片先用卢戈氏碘或偏高碘酸钠预处理，以增强免疫反应性。α-SMA在每个卵泡的索状细胞、白膜和外膜中得到证实。这些结构相互连接，形成一个卵巢悬韧带装置。灯刷前期卵泡的膜细胞也表达α-SMA。在排卵前卵泡壁中，结蛋白存在于索状细胞和白膜细胞以及外膜的少数细胞中。在内膜中，在紧邻血窦内皮的细胞中观察到结蛋白，有时也观察到α-SMA，这些细胞可能是周细胞。我们的结果支持这样一种假说，即鸟类的卵巢卵泡拥有一个膜收缩系统，该系统可能参与排卵过程。
