Goodridge Carolyn F, Beaudry Randolph M, Pestka James J, Smith Denise M
Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824, USA.
J Agric Food Chem. 2003 Jul 16;51(15):4185-90. doi: 10.1021/jf0260646.
A method using solid phase microextraction (SPME) combined with gas chromatography/mass spectrometry (GC/MS) was developed and used to determine the oxidation of freeze-dried chicken myofibrils spiked with methyl linoleate. Freeze-dried chicken myofibrils were found to act as a significant reservoir for hexanal. Recovery of hexanal emissions from the headspace above spiked myofibrils was 95% using a 5 min sampling time, with a total analysis time of approximately 12 min/sample. The SPME-GC/MS working linear response was from 0.01 to 10 mg hexanal/L (r( 2) = 0.995). Freeze-dried chicken myofibrils with added methyl linoleate (0.6 mmol/g of protein) were stored at 50 degrees C at water activities of 0.30 and 0.75 for 0, 12, 27, and 50 h. Lipid oxidation was determined using SPME-GC/MS to measure headspace hexanal concentration, the thiobarbituric acid reactive substances assay (TBARS) to quantify malonaldehyde, and a conjugated diene assay. Lipid oxidation was influenced by storage time and water activity. A strong correlation (r = 0.938) existed between SPME-GC/MS and TBARS. The use of SPME-GC/MS was a sensitive and rapid method for detecting hexanal as an indicator of lipid oxidation in chicken myofibrils.
开发了一种使用固相微萃取（SPME）结合气相色谱/质谱（GC/MS）的方法，并用于测定添加亚油酸甲酯的冻干鸡肉肌原纤维的氧化情况。发现冻干鸡肉肌原纤维是己醛的重要储存库。使用5分钟采样时间，从添加肌原纤维上方顶空中回收己醛排放物的回收率为95%，每个样品的总分析时间约为12分钟。SPME-GC/MS的工作线性响应为0.01至10 mg己醛/升（r(2)=0.995）。添加亚油酸甲酯（0.6 mmol/g蛋白质）的冻干鸡肉肌原纤维在50℃、水分活度为0.30和0.75的条件下储存0、12、27和50小时。使用SPME-GC/MS测量顶空己醛浓度、硫代巴比妥酸反应物质测定法（TBARS）定量丙二醛以及共轭二烯测定法来测定脂质氧化。脂质氧化受储存时间和水分活度的影响。SPME-GC/MS与TBARS之间存在很强的相关性（r = 0.938）。使用SPME-GC/MS是检测己醛作为鸡肉肌原纤维脂质氧化指标的灵敏且快速的方法。
