Pei X F, Leigh I M, Watt F M
Department of Pathology, Georgetown University Medical School, Washington, DC.
Epithelial Cell Biol. 1992 Apr;1(2):84-9.
We have used immunoblotting to compare expression of type I keratins in two strains of normal human epidermal keratinocytes (v and u) and their HPV16-transformed derivatives (vp and up). The levels of keratins 14 and 17 were similar in all four cell strains, whereas keratins 18 and 19 were more abundant in vp and up than in the normal parental keratinocytes. Keratin 13 was more abundant in the transformed cells than in the parentals; in addition, expression in v was higher than in u, and expression in vp was higher than in up, suggesting strain-specific variation in expression. Keratin 16 was the only keratin to be more highly expressed in v and u than in vp and up; this is consistent with the reduced capacity of the transformants for stratification and terminal differentiation. Double-label immunofluorescence of vp and up showed that more cells expressed involucrin than keratin 16. We conclude that HPV16 transformation results in marked changes in keratin expression. The increased expression of keratin 18, a keratin that is normally expressed in simple epithelia, fits well with reports of increased keratin 18 expression in invasive squamous cell carcinomas of skin and other keratinocyte-derived tumours.
我们利用免疫印迹法比较了两株正常人表皮角质形成细胞（v株和u株）及其HPV16转化衍生物（vp株和up株）中I型角蛋白的表达情况。在所有四株细胞系中，角蛋白14和17的水平相似，而角蛋白18和19在vp株和up株中比在正常亲代角质形成细胞中更为丰富。角蛋白13在转化细胞中比在亲代细胞中更为丰富；此外，v株中的表达高于u株，vp株中的表达高于up株，提示存在表达的菌株特异性差异。角蛋白16是唯一在v株和u株中比在vp株和up株中表达更高的角蛋白；这与转化体分层和终末分化能力降低一致。vp株和up株的双标记免疫荧光显示，表达内聚蛋白的细胞比表达角蛋白16的细胞更多。我们得出结论，HPV16转化导致角蛋白表达发生显著变化。角蛋白18通常在单层上皮中表达，其表达增加与皮肤浸润性鳞状细胞癌和其他角质形成细胞源性肿瘤中角蛋白18表达增加的报道相符。
