• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

关于细胞表面蛋白酶结构构象变化的观察，以及随后其被竞争性和非竞争性抑制剂识别的能力。

Observations on the conformational changes in the structure of a cell surface protease, followed by its ability to be recognised by competitive and non-competitive inhibitors.

作者信息

Steven F S, Griffin M M, Anees M, Benbow E W

机构信息

Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, UK.

出版信息

J Enzyme Inhib. 1992;6(3):251-8. doi: 10.3109/14756369209020175.

DOI:10.3109/14756369209020175
PMID:1284962
Abstract

Lung tumour cells possess a cell surface protease which can be inhibited by a cytoplasmic protein inhibitor extracted from these cells. The dissociation of this enzyme-inhibitor complex on the surface of tumour cells in sections treated with 10(-4) M sodium dodecyl sulphate has been studied. The dissociation of the inhibitor and regain of enzymic activity was followed by the use of a fluorescent probe which binds to the active centre of the cell surface enzyme in a competitive manner.

摘要

肺肿瘤细胞具有一种细胞表面蛋白酶，这种蛋白酶可被从这些细胞中提取的一种细胞质蛋白抑制剂所抑制。已经研究了用10⁻⁴ M十二烷基硫酸钠处理的切片中肿瘤细胞表面这种酶 - 抑制剂复合物的解离情况。通过使用一种荧光探针来跟踪抑制剂的解离和酶活性的恢复，该荧光探针以竞争方式与细胞表面酶的活性中心结合。

相似文献

1
Observations on the conformational changes in the structure of a cell surface protease, followed by its ability to be recognised by competitive and non-competitive inhibitors.关于细胞表面蛋白酶结构构象变化的观察，以及随后其被竞争性和非竞争性抑制剂识别的能力。
J Enzyme Inhib. 1992;6(3):251-8. doi: 10.3109/14756369209020175.
2
Studies on the interaction and exchange of inhibitors and proteases on the surface of tumour cells in frozen sections.关于冷冻切片中肿瘤细胞表面抑制剂与蛋白酶相互作用及交换的研究。
Anticancer Res. 1991 Nov-Dec;11(6):2117-22.
3
The role of fibrin fibrils in the dissociation of a cell surface protease-inhibitor complex and evidence for the recapture of the inhibitor protein.纤维蛋白原纤维在细胞表面蛋白酶 - 抑制剂复合物解离中的作用及抑制剂蛋白重新捕获的证据。
J Enzyme Inhib. 1992;5(4):299-315. doi: 10.3109/14756369109069072.
4
Differential competitive inhibition of a cell surface protease on normal epithelial cells and carcinoma cells of the colon.细胞表面蛋白酶对结肠正常上皮细胞和癌细胞的差异竞争性抑制作用。
Anticancer Res. 1994 Sep-Oct;14(5A):2013-6.
5
Temporary competitive inhibition of a tumour cell surface protease as a protective mechanism in the preparation of the membrane bound native enzyme in the presence of excess cytoplasmic inhibitors.
J Enzyme Inhib. 1991;5(1):77-85. doi: 10.3109/14756369109069061.
6
Association and dissociation of a protease and its inhibitor on the surface of lung squamous cell carcinoma cells.蛋白酶与其抑制剂在肺鳞状细胞癌细胞表面的结合与解离
Anticancer Res. 1993 Jul-Aug;13(4):1063-8.
7
Competitive inhibition of a tumour cell surface protease. A rapid technique for in vitro testing of selective targeting systems.肿瘤细胞表面蛋白酶的竞争性抑制。一种用于体外测试选择性靶向系统的快速技术。
J Enzyme Inhib. 1990;4(1):63-73. doi: 10.3109/14756369009030390.
8
The inhibitor reacting with a tumour cell surface protease can be exchanged with plasminogen activator inhibitor (PAI-1).与肿瘤细胞表面蛋白酶发生反应的抑制剂可与纤溶酶原激活物抑制剂（PAI-1）进行交换。
J Enzyme Inhib. 1991;4(3):273-9. doi: 10.3109/14756369109035851.
9
Retinoic acid inhibition of a tumour protease immobilised on cell surfaces and in free solution.维甲酸对固定于细胞表面及游离溶液中的肿瘤蛋白酶的抑制作用。
J Enzyme Inhib. 1994;8(1):51-9. doi: 10.3109/14756369409040776.
10
Fluorescent location of human colonic tumour cells by means of an enzyme-inhibitor complex.通过酶抑制剂复合物对人结肠肿瘤细胞进行荧光定位。
J Enzyme Inhib. 1990;3(3):229-36. doi: 10.3109/14756369009035841.