Yang W I
Department of Pathology, Yonsei University Wonju College of Medicine, Korea.
Yonsei Med J. 1992 Dec;33(4):309-19. doi: 10.3349/ymj.1992.33.4.309.
Immunohistochemical study on 26 cases of Langerhans cell histiocytosis (LCH) using several leukocyte antibodies in addition to traditionally used markers (S-100 protein and peanut agglutinin) revealed that the proliferating cells of LCH expressed UCHL1, MT1 as well as classically known positivity for S-100 protein, HLA-DR and peanut agglutinin but were negative for OPD4. In comparison to S-100 protein peanut agglutinin (PNA) using a two stage method produced weaker staining and positively stained cells were sparse. Also in this study, a small proportion of proliferating cells in LCH was observed to be reactive for both myeloid/macrophage antigens (KPI, MAC 387 and lysozyme) and Langerhans cell marker (S-100 protein), verifying the existence of a hybrid form of histiocytes.
除了传统使用的标志物（S-100蛋白和花生凝集素）外，使用几种白细胞抗体对26例朗格汉斯细胞组织细胞增多症（LCH）进行免疫组织化学研究发现，LCH的增殖细胞表达UCHL1、MT1，以及经典已知的S-100蛋白、HLA-DR和花生凝集素阳性，但OPD4呈阴性。与S-100蛋白相比，采用两步法的花生凝集素（PNA）染色较弱，阳性染色细胞稀少。同样在本研究中，观察到LCH中有一小部分增殖细胞对髓系/巨噬细胞抗原（KPI、MAC 387和溶菌酶）和朗格汉斯细胞标志物（S-100蛋白）均有反应，证实了存在一种混合形式的组织细胞。
