Moore J M, Shi Y P, Othoro C, Nahlen B L, Lal A A, Udhayakumar V
Center for Tropical and Emerging Global Diseases, and Department of Medical Microbiology and Parasitology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Placenta. 2003 Aug;24(7):779-85. doi: 10.1016/s0143-4004(03)00112-7.
Understanding maternal immune responses in the placenta is critical for management of pregnancy failures and haematogenous infections during pregnancy. However, it is unknown whether maternal placental intervillous blood (IVB) mononuclear cell populations are distinct from those found in maternal peripheral blood (PB). In this study, cell populations in the IVB and PB from immediate postpartum women were compared by flow cytometry. While levels of B and CD4+ and CD8+ T lymphocytes were similar, IVB contained significantly higher levels of monocytes (10.9+/-5.9 versus 5.5+/-2.5 per cent, respectively) and natural killer cells (14.3+/-9.6 versus 5.9+/-3.2 per cent, respectively) than the PB. Expression of the early activation marker CD69 was increased on T cells in the IVB, whereas levels of HLA-DR, a late activation marker, were similar between IVB and PB. These results suggest that maternal cells that circulate through the intervillous compartment may be subject to local influences that affect their distribution, phenotype and function. Further comparative study of these blood compartments will be necessary to elucidate the mechanisms by which the local placental milieu influences the IVB.
了解胎盘母体免疫反应对于处理妊娠失败和孕期血源性感染至关重要。然而，尚不清楚母体胎盘绒毛间隙血（IVB）中的单核细胞群体是否与母体外周血（PB）中的不同。在本研究中，通过流式细胞术比较了产后即刻女性IVB和PB中的细胞群体。虽然B细胞、CD4 +和CD8 + T淋巴细胞水平相似，但IVB中的单核细胞（分别为10.9±5.9%和5.5±2.5%）和自然杀伤细胞（分别为14.3±9.6%和5.9±3.2%）水平显著高于PB。IVB中T细胞上早期激活标志物CD69的表达增加，而晚期激活标志物HLA - DR的水平在IVB和PB之间相似。这些结果表明，循环通过绒毛间隙的母体细胞可能受到影响其分布、表型和功能的局部影响。有必要对这些血腔进行进一步的比较研究，以阐明局部胎盘环境影响IVB的机制。
