Saito H, Kagawa T, Tada S, Tsunematsu S, Guevara F M, Watanabe T, Morizane T, Tsuchiya M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Cancer Biochem Biophys. 1992 Nov;13(2):75-84.
The effects of agents which are known to be differentiation inducers on a human hepatoma cell line PLC/PRF/5 were investigated. Dexamethasone (DEX), sodium butyrate (SB) or dimethylsulfoxide (DMSO) were examined. They all reduced cell proliferation but differ from each other in effect on the secretion of alphafetoprotein (AFP) and hepatitis B surface antigen (HBsAg), changes in morphology and RNA transcription. SB changed the cell from polygonal into a fibroblast-like type and decreased AFP secretion. DMSO decreased the cell size and changed AFP secretion in the same manner as SB. DEX changed the cell into a larger size, as well as increased AFP secretion. HBsAg secretion and also HB virus DNA transcription was enhanced by 3 agents. AFP and myc gene transcriptions were reduced by SB but DMSO reduced only AFP. Albumin gene transcription was enhanced by SB and DEX. These results indicate that the decrease of PLC/PRF/5 proliferation is induced through different mechanisms by these 3 agents.
研究了已知的分化诱导剂对人肝癌细胞系PLC/PRF/5的作用。检测了地塞米松（DEX）、丁酸钠（SB）或二甲基亚砜（DMSO）。它们均能降低细胞增殖，但在对甲胎蛋白（AFP）和乙型肝炎表面抗原（HBsAg）分泌的影响、形态变化以及RNA转录方面彼此不同。SB使细胞从多边形变为成纤维细胞样类型，并减少AFP分泌。DMSO减小细胞大小，并以与SB相同的方式改变AFP分泌。DEX使细胞变大，并增加AFP分泌。三种试剂均增强了HBsAg分泌以及HB病毒DNA转录。SB可降低AFP和myc基因转录，但DMSO仅降低AFP。SB和DEX可增强白蛋白基因转录。这些结果表明，这三种试剂通过不同机制诱导PLC/PRF/5增殖的降低。
