Chaisavaneeyakorn Sujittra, Moore Julie M, Mirel Lisa, Othoro Caroline, Otieno Juliana, Chaiyaroj Sansanee C, Shi Ya Ping, Nahlen Bernard L, Lal Altaf A, Udhayakumar Venkatachalam
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333, USA.
Clin Diagn Lab Immunol. 2003 Jul;10(4):631-6. doi: 10.1128/cdli.10.4.631-636.2003.
Macrophage inflammatory protein-1 alpha (MIP-1 alpha) and MIP-1 beta play an important role in modulating immune responses. To understand their importance in immunity to placental malaria (PM) and in human immunodeficiency virus (HIV)-PM coinfection, we investigated levels of these chemokines in the placental intervillous blood plasma (IVB plasma) and cord blood plasma of HIV-negative PM-negative, HIV-negative PM-positive, HIV-positive PM-negative, and HIV-positive PM-positive women. Compared to HIV-negative PM-negative women, the MIP-1 beta concentration in IVB plasma was significantly elevated in HIV-negative PM-positive women and HIV-positive PM-positive women, but it was unaltered in HIV-positive PM-negative women. Also, PM-infected women, irrespective of their HIV status, had significantly higher levels of MIP-1 beta than HIV-positive PM-negative women. The MIP-1 alpha level was not altered in association with either infection. The IVB plasma levels of MIP-1 alpha and MIP-1 beta positively correlated with the cord blood plasma levels of these chemokines. As with IVB plasma, only cord plasma from PM-infected mothers had significantly elevated levels of MIP-1 beta compared to PM-negative mothers, irrespective of their HIV infection status. MIP-1 beta and MIP-1 alpha levels in PM-positive women were positively associated with parasite density and malaria pigment levels. Regardless of HIV serostatus, the IVB MIP-1 beta level was significantly lower in women with PM-associated anemia. In summary, an elevated level of MIP-1 beta was associated with PM. HIV infection did not significantly alter these two chemokine levels in IVB plasma.
巨噬细胞炎性蛋白-1α（MIP-1α）和MIP-1β在调节免疫反应中发挥重要作用。为了解它们在胎盘疟疾（PM）免疫及人类免疫缺陷病毒（HIV）-PM合并感染中的重要性，我们调查了HIV阴性PM阴性、HIV阴性PM阳性、HIV阳性PM阴性及HIV阳性PM阳性女性的胎盘绒毛间隙血浆（IVB血浆）和脐血血浆中这些趋化因子的水平。与HIV阴性PM阴性女性相比，HIV阴性PM阳性女性和HIV阳性PM阳性女性IVB血浆中的MIP-1β浓度显著升高，但HIV阳性PM阴性女性未发生改变。此外，无论HIV感染状态如何，PM感染女性的MIP-1β水平均显著高于HIV阳性PM阴性女性。MIP-1α水平未因任何一种感染而发生改变。MIP-1α和MIP-1β的IVB血浆水平与这些趋化因子的脐血血浆水平呈正相关。与IVB血浆情况相同，无论HIV感染状态如何，仅来自PM感染母亲的脐血血浆中MIP-1β水平相较于PM阴性母亲显著升高。PM阳性女性的MIP-1β和MIP-1α水平与寄生虫密度和疟色素水平呈正相关。无论HIV血清学状态如何，PM相关性贫血女性的IVB MIP-1β水平显著较低。总之，MIP-1β水平升高与PM相关。HIV感染并未显著改变IVB血浆中这两种趋化因子的水平。
