Young C, Jarrell B E, Hoying J B, Williams S K
Department of Surgery, Jefferson Medical College, Philadelphia, PA 19107.
Cell Transplant. 1992;1(4):293-8. doi: 10.1177/096368979200100406.
The transplantation of endothelial cells represents a technology which has been suggested for applications ranging from improvement in function of implanted vascular devices to genetic therapy. The use of microvascular endothelial cell transplantation has seen increased use both in animal studies as well as clinical use. This report describes our techniques for the isolation and establishment of initial cultures of microvascular endothelial cells derived from porcine fat. A variety of anatomic sites within the pig were evaluated to determine the appropriateness of different sources of fat for endothelial cell isolation. The properitoneal fat was determined to be optimal due to the predominance of endothelium in this tissue and the ease of isolation of microvascular endothelium following collagenase digestion. The study of endothelial cell transplantation in the porcine model is now possible using the methods described for adipose tissue-derived microvessel endothelial cell isolation.
内皮细胞移植是一项已被提出可用于多种应用的技术，这些应用范围从改善植入式血管装置的功能到基因治疗。微血管内皮细胞移植在动物研究和临床应用中都有越来越多的使用。本报告描述了我们从猪脂肪中分离和建立微血管内皮细胞原代培养物的技术。对猪体内的各种解剖部位进行了评估，以确定不同脂肪来源用于内皮细胞分离的适宜性。由于该组织中内皮细胞占优势，且胶原酶消化后易于分离微血管内皮细胞，因此确定腹膜脂肪是最佳的。使用所述的脂肪组织来源的微血管内皮细胞分离方法，现在可以在猪模型中进行内皮细胞移植研究。
