拟南芥中 CpG 甲基化的消除会改变异染色质中组蛋白 H3 甲基化的模式。
Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin.
作者信息
Tariq Muhammad, Saze Hidetoshi, Probst Aline V, Lichota Jacek, Habu Yoshiki, Paszkowski Jerzy
机构信息
Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.
出版信息
Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8823-7. doi: 10.1073/pnas.1432939100. Epub 2003 Jul 9.
Abstract
In mammals and plants, formation of heterochromatin is associated with hypermethylation of DNA at CpG sites and histone H3 methylation at lysine 9. Previous studies have revealed that maintenance of DNA methylation in Neurospora and Arabidopsis requires histone H3 methylation. A feedback loop from DNA methylation to histone methylation, however, is less understood. Its recent examination in Arabidopsis with a partial loss of function in DNA methyltransferase 1 (responsible for maintenance of CpG methylation) yielded conflicting results. Here we report that complete removal of CpG methylation in an Arabidopsis mutant null for DNA maintenance methyltransferase results in a clear loss of histone H3 methylation at lysine 9 in heterochromatin and also at heterochromatic loci that remain transcriptionally silent. Surprisingly, these dramatic alterations are not reflected in heterochromatin relaxation.
摘要
在哺乳动物和植物中，异染色质的形成与CpG位点处DNA的高甲基化以及赖氨酸9处组蛋白H3的甲基化有关。先前的研究表明，粗糙脉孢菌和拟南芥中DNA甲基化的维持需要组蛋白H3甲基化。然而，从DNA甲基化到组蛋白甲基化的反馈回路却鲜为人知。最近在拟南芥中对DNA甲基转移酶1（负责维持CpG甲基化）功能部分丧失的研究产生了相互矛盾的结果。在此我们报告，在一个DNA维持甲基转移酶缺失的拟南芥突变体中，CpG甲基化的完全去除导致异染色质中赖氨酸9处组蛋白H3甲基化明显丧失，在那些仍保持转录沉默的异染色质位点也是如此。令人惊讶的是，这些显著变化并未反映在异染色质的松弛上。
相似文献
1
Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin.拟南芥中 CpG 甲基化的消除会改变异染色质中组蛋白 H3 甲基化的模式。
Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8823-7. doi: 10.1073/pnas.1432939100. Epub 2003 Jul 9.
2
Histone modifications in Arabidopsis- high methylation of H3 lysine 9 is dispensable for constitutive heterochromatin.拟南芥中的组蛋白修饰——H3赖氨酸9的高甲基化对于组成型异染色质而言并非必需。
Plant J. 2003 Feb;33(3):471-80. doi: 10.1046/j.1365-313x.2003.01638.x.
3
Interplay between two epigenetic marks. DNA methylation and histone H3 lysine 9 methylation.两种表观遗传标记之间的相互作用。DNA甲基化和组蛋白H3赖氨酸9甲基化。
Curr Biol. 2002 Aug 20;12(16):1360-7. doi: 10.1016/s0960-9822(02)00976-4.
4
Distinct regulation of histone H3 methylation at lysines 27 and 9 by CpG methylation in Arabidopsis.拟南芥中CpG甲基化对赖氨酸27和9处组蛋白H3甲基化的不同调控
EMBO J. 2005 Aug 3;24(15):2783-91. doi: 10.1038/sj.emboj.7600743. Epub 2005 Jul 7.
5
Dependence of heterochromatic histone H3 methylation patterns on the Arabidopsis gene DDM1.异染色质组蛋白H3甲基化模式对拟南芥基因DDM1的依赖性。
Science. 2002 Sep 13;297(5588):1871-3. doi: 10.1126/science.1074950. Epub 2002 Jun 20.
6
Control of CpNpG DNA methylation by the KRYPTONITE histone H3 methyltransferase.由“氪石”组蛋白H3甲基转移酶调控CpNpG DNA甲基化
Nature. 2002 Apr 4;416(6880):556-60. doi: 10.1038/nature731. Epub 2002 Mar 17.
7
Loss of the DNA methyltransferase MET1 Induces H3K9 hypermethylation at PcG target genes and redistribution of H3K27 trimethylation to transposons in Arabidopsis thaliana.DNA 甲基转移酶 MET1 的缺失会导致拟南芥 PcG 靶基因的 H3K9 过度甲基化和 H3K27 三甲基化向转座子的重新分布。
PLoS Genet. 2012;8(11):e1003062. doi: 10.1371/journal.pgen.1003062. Epub 2012 Nov 29.
8
Histone H1 prevents non-CG methylation-mediated small RNA biogenesis in heterochromatin.组蛋白 H1 可防止异染色质中非 CG 甲基化介导的小 RNA 生物发生。
Elife. 2021 Dec 1;10:e72676. doi: 10.7554/eLife.72676.
9
Three SRA-domain methylcytosine-binding proteins cooperate to maintain global CpG methylation and epigenetic silencing in Arabidopsis.三种含SRA结构域的甲基胞嘧啶结合蛋白协同作用，以维持拟南芥全基因组的CpG甲基化和表观遗传沉默。
PLoS Genet. 2008 Aug 15;4(8):e1000156. doi: 10.1371/journal.pgen.1000156.
10
The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana.组蛋白H3变体H3.3调控拟南芥基因体DNA甲基化。
Genome Biol. 2017 May 18;18(1):94. doi: 10.1186/s13059-017-1221-3.
引用本文的文献
1
BpWRKY6 regulates insect resistance by affecting jasmonic acid and terpenoid synthesis in Betula platyphylla.BpWRKY6通过影响白桦中茉莉酸和萜类化合物的合成来调节抗虫性。
Plant Biotechnol J. 2025 Sep;23(9):3682-3696. doi: 10.1111/pbi.70169. Epub 2025 Jun 10.
2
Partial redundancy buffers deleterious effects of mutating DNA methyltransferase 1-1 (MET1-1) in polyploid wheat.部分冗余缓冲了多倍体小麦中DNA甲基转移酶1-1（MET1-1）突变的有害影响。
J Exp Bot. 2025 Jun 17;76(9):2500-2516. doi: 10.1093/jxb/eraf016.
3
REDD1 expression in podocytes facilitates renal inflammation and pyroptosis in streptozotocin-induced diabetic nephropathy.足细胞中REDD1的表达促进链脲佐菌素诱导的糖尿病肾病中的肾脏炎症和细胞焦亡。
Cell Death Dis. 2025 Feb 7;16(1):79. doi: 10.1038/s41419-025-07396-4.
4
Estrogen-related receptor alpha promotes thyroid tumor cell survival via a tumor subtype-specific regulation of target gene networks.雌激素相关受体 alpha 通过肿瘤亚型特异性调节靶基因网络促进甲状腺肿瘤细胞存活。
Oncogene. 2024 Jul;43(31):2431-2446. doi: 10.1038/s41388-024-03078-1. Epub 2024 Jun 27.
5
Regulation of heterochromatin organization in plants.植物异染色质组织的调控。
J Plant Res. 2024 Sep;137(5):685-693. doi: 10.1007/s10265-024-01550-3. Epub 2024 Jun 24.
6
Non-canonical bases differentially represented in the sex chromosomes of the dioecious plant Silene latifolia.在雌雄异株植物石竹属中不同表达的非规范碱基。
J Exp Bot. 2024 Jul 10;75(13):3849-3861. doi: 10.1093/jxb/erae178.
7
Set1/COMPASS regulates growth, pathogenicity, and patulin biosynthesis of Penicillium expansum via H3K4 methylation and the interaction with PeVelB.Set1/COMPASS 通过 H3K4 甲基化和与 PeVelB 的相互作用调控扩展青霉的生长、致病性和棒曲霉素生物合成。
J Adv Res. 2024 Aug;62:47-57. doi: 10.1016/j.jare.2023.10.001. Epub 2023 Oct 4.
8
Histone H3K4 Methyltransferase PeSet1 Regulates Colonization, Patulin Biosynthesis, and Stress Responses of .组蛋白 H3K4 甲基转移酶 PeSet1 调控. 的定植、棒曲霉素生物合成和应激反应
Microbiol Spectr. 2023 Feb 14;11(1):e0354522. doi: 10.1128/spectrum.03545-22. Epub 2023 Jan 12.
9
Canalization of genome-wide transcriptional activity in Arabidopsis thaliana accessions by MET1-dependent CG methylation.通过 MET1 依赖性 CG 甲基化实现拟南芥品系中全基因组转录活性的 canalization。
Genome Biol. 2022 Dec 20;23(1):263. doi: 10.1186/s13059-022-02833-5.
10
The Arabidopsis APOLO and human UPAT sequence-unrelated long noncoding RNAs can modulate DNA and histone methylation machineries in plants.拟南芥 APOLO 和人类 UPAT 序列无关的长非编码 RNA 可以在植物中调节 DNA 和组蛋白甲基化机制。
Genome Biol. 2022 Aug 29;23(1):181. doi: 10.1186/s13059-022-02750-7.
本文引用的文献
1
Methyl-CpG binding domain 1 (MBD1) interacts with the Suv39h1-HP1 heterochromatic complex for DNA methylation-based transcriptional repression.甲基化CpG结合结构域1（MBD1）与Suv39h1-HP1异染色质复合物相互作用，以实现基于DNA甲基化的转录抑制。
J Biol Chem. 2003 Jun 27;278(26):24132-8. doi: 10.1074/jbc.M302283200. Epub 2003 Apr 23.
2
Trimethylated lysine 9 of histone H3 is a mark for DNA methylation in Neurospora crassa.组蛋白H3的赖氨酸9位点三甲基化是粗糙脉孢菌中DNA甲基化的一个标记。
Nat Genet. 2003 May;34(1):75-9. doi: 10.1038/ng1143.
3
Maintenance of CpG methylation is essential for epigenetic inheritance during plant gametogenesis.在植物配子发生过程中，CpG甲基化的维持对于表观遗传遗传至关重要。
Nat Genet. 2003 May;34(1):65-9. doi: 10.1038/ng1138.
4
Arabidopsis MET1 cytosine methyltransferase mutants.拟南芥MET1胞嘧啶甲基转移酶突变体。
Genetics. 2003 Mar;163(3):1109-22. doi: 10.1093/genetics/163.3.1109.
5
Two means of transcriptional reactivation within heterochromatin.异染色质内转录重新激活的两种方式。
Plant J. 2003 Feb;33(4):743-9. doi: 10.1046/j.1365-313x.2003.01667.x.
6
Histone modifications in Arabidopsis- high methylation of H3 lysine 9 is dispensable for constitutive heterochromatin.拟南芥中的组蛋白修饰——H3赖氨酸9的高甲基化对于组成型异染色质而言并非必需。
Plant J. 2003 Feb;33(3):471-80. doi: 10.1046/j.1365-313x.2003.01638.x.
7
ARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation.AGO4对基因座特异性小干扰RNA积累以及DNA和组蛋白甲基化的调控
Science. 2003 Jan 31;299(5607):716-9. doi: 10.1126/science.1079695. Epub 2003 Jan 9.
8
DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis.DNA甲基化调控拟南芥中组蛋白H3赖氨酸9的甲基化及异染色质组装。
EMBO J. 2002 Dec 2;21(23):6549-59. doi: 10.1093/emboj/cdf657.
9
The methyl-CpG-binding protein MeCP2 links DNA methylation to histone methylation.甲基化CpG结合蛋白MeCP2将DNA甲基化与组蛋白甲基化联系起来。
J Biol Chem. 2003 Feb 7;278(6):4035-40. doi: 10.1074/jbc.M210256200. Epub 2002 Nov 9.
10
Two regulatory levels of transcriptional gene silencing in Arabidopsis.拟南芥中转录基因沉默的两个调控水平。
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13659-62. doi: 10.1073/pnas.202380499. Epub 2002 Oct 7.
Zotero 插件