Jeske W, Zgliczyński W, Zgliczyński S, Kasperlik-Załuska A, Stopińska U, Soszyński P, Zieliński M
Dept. of Endocrinology, Center of Postgraduate Medical Education, Warsaw.
Endokrynol Pol. 1992;43(3):250-4.
The radioimmunoassay of alpha-subunit adapted in our laboratory was widely evaluated. Three different antisera (anti-pituitary alpha-subunit, anti-alpha-TSH and anti-alpha-hCG), the labelled preparations of pituitary alpha-subunit and alpha-hCG, and cross-reactivity with intact glycoprotein hormones (MRC standards of LH, FSH, hCG and TSH) were tested for their potential influence on the results of the assay. The basal levels of alpha-subunit were measured in 48 healthy young men, 48 normally menstruating women, 33 menopausal women, 37 pregnant women and 70 patients with pituitary adenoma. In addition a possibility of pulsatile secretion of alpha-subunit was investigated in 9 healthy young women, the ranges of alpha-subunit concentrations found were as follow (means +/- SD): 1.2 +/- 0.4 micrograms/l--in young men, 1.1 +/- 0.4 micrograms/l--in young women, 3.2 +/- 0.7 micrograms/l--in postmenopausal women, 1-54 micrograms/l--in pregnant women, and between 2.6 and 44.0 micrograms/l in 14 of 70 patients with pituitary adenoma. There were good correlations of results for 3 different antisera and their cross-reactivity with LH, FSH, hCG and TSH were just as low. In conclusion, the alpha-subunit assay appears clinically useful and should be widely applied in routine endocrinological diagnostics.
对我们实验室采用的α亚基放射免疫测定法进行了广泛评估。测试了三种不同的抗血清（抗垂体α亚基、抗α促甲状腺激素和抗α人绒毛膜促性腺激素）、垂体α亚基和α人绒毛膜促性腺激素的标记制剂，以及它们与完整糖蛋白激素（促黄体生成素、促卵泡激素、人绒毛膜促性腺激素和促甲状腺激素的MRC标准品）的交叉反应性对测定结果的潜在影响。测定了48名健康年轻男性、48名月经正常的女性、33名绝经后女性、37名孕妇和70名垂体腺瘤患者的α亚基基础水平。此外，还对9名健康年轻女性进行了α亚基脉冲分泌可能性的研究，发现的α亚基浓度范围如下（均值±标准差）：年轻男性为1.2±0.4微克/升，年轻女性为1.1±0.4微克/升，绝经后女性为3.2±0.7微克/升，孕妇为1 - 54微克/升，70名垂体腺瘤患者中有14名患者的α亚基浓度在2.6至44.0微克/升之间。三种不同抗血清的结果相关性良好，它们与促黄体生成素、促卵泡激素、人绒毛膜促性腺激素和促甲状腺激素的交叉反应性同样较低。总之，α亚基测定在临床上似乎有用，应广泛应用于常规内分泌诊断。
