Ono K, Nakane H, Fukushima M, Chermann J C, Barré-Sinoussi F
Laboratory of Viral Oncology, Aichi Cancer Center Research Institute, Nagoya, Japan.
Biomed Pharmacother. 1990;44(1):13-6. doi: 10.1016/0753-3322(90)90063-f.
A traditional Kampo drug, Sho-saiko-to, composed of several herb extracts, differentially inhibited the activities of reverse transcriptase and human cellular DNA polymerase alpha and beta. Reverse transcriptases from murine leukemia virus and human immunodeficiency virus were inhibited by over 80% and 50%, respectively, in the presence of 100 micrograms/ml Sho-saiko-to, whereas DNA polymerase alpha was much less sensitive to inhibition by this drug than were the reverse transcriptases. DNA polymerase gamma was not inhibited by this drug at concentrations of up to 500 micrograms/ml. Only DNA polymerase beta was moderately inhibited by Sho-saiko-to. Thus, it has been shown that the inhibition by Sho-saiko-to is relatively specific for reverse transcriptase and that the drug contains as yet unidentified inhibitory substance(s) for reverse transcriptase.
一种传统的汉方药，小柴胡汤，由几种草药提取物组成，能不同程度地抑制逆转录酶以及人细胞DNA聚合酶α和β的活性。在存在100微克/毫升小柴胡汤的情况下，来自鼠白血病病毒和人类免疫缺陷病毒的逆转录酶分别被抑制80%以上和50%，而DNA聚合酶α对该药物抑制的敏感性远低于逆转录酶。在浓度高达500微克/毫升时，DNA聚合酶γ未被该药物抑制。只有DNA聚合酶β受到小柴胡汤的中度抑制。因此，已表明小柴胡汤的抑制作用对逆转录酶相对具有特异性，并且该药物含有尚未确定的逆转录酶抑制物质。
