[小鼠胚胎成纤维细胞与人胚胎成纤维细胞生物学特性的比较]
[The comparison of biologic character between mouse embryonic fibroblast and human embryonic fibroblast].
作者信息
Zhang Yi, Zhao Liansan, Wang Chengxiao, Lei Binjun
机构信息
Infectious Diseases Department, West China Hospital of Sichuan University, Key Laboratory of Molecular Biology for Infectious Diseases of Sichuan Province, Chengdu 610041.
出版信息
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2003 Jun;20(2):251-4.
To evaluate the feasibility of using human embryonic fibroblast(HEF) as feeder layer in the culture of human embryonic stem(ES) cells in vitro, we investigated the morphology, the sensitivity to 0.25% trypsin, the growth curve and cell cycle of HEF with DMEM(low glucose) +10% FBS used as culture medium, and then we compared HEF with mouse embryonic fibroblast (MEF). The results showed that both HEF and MEF are adherent cells in vitro, and HEF has longer life span and better growth ability than MEF. In room temperature, HEF is more sensitive to 0.25% trypsin. Our research suggested that HEF can be used as feeder layer in culture of ES cells. HEF has longer service life than MEF and is worthy to be studied further.
为评估在体外培养人胚胎干细胞时使用人胚胎成纤维细胞（HEF）作为饲养层的可行性，我们以DMEM（低糖）+10%胎牛血清作为培养基，研究了HEF的形态、对0.25%胰蛋白酶的敏感性、生长曲线和细胞周期，然后将HEF与小鼠胚胎成纤维细胞（MEF）进行比较。结果表明，HEF和MEF在体外均为贴壁细胞，且HEF比MEF具有更长的寿命和更好的生长能力。在室温下，HEF对0.25%胰蛋白酶更敏感。我们的研究表明，HEF可作为胚胎干细胞培养的饲养层。HEF比MEF具有更长的使用寿命，值得进一步研究。
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