Tian Hai-Bin, Wang Hong, Sha Hong-Ying, Xu Xu-Jun, Zhu Min, Wu You-Bing, Cheng Sheng-Hua, Chen Jian-Quan, Shi Yong-Xiang, Bai Zeng-Liang, Cheng Guo-Xiang
Shanghai Transgenic Research Center, Shanghai 201203, China.
Cell Biol Int. 2006 May;30(5):452-8. doi: 10.1016/j.cellbi.2006.02.006. Epub 2006 Mar 6.
Goat embryonic stem (ES)-like cells could be isolated from primary materials-inner cell masses (ICMs) and remain undifferentiated for eight passages in a new culture system containing mouse ES cell conditioned medium (ESCCM) and on a feeder layer of mouse embryo fibroblasts (MEFs). However, when cultured in medium without mouse ESCCM, goat ES-like cells could not survive for more than three passages. In addition, no ES-like cells could be obtained when ICMs were cultured on goat embryo fibroblasts or the primary materials-whole goat blastocysts were cultured on MEFs. Goat ES-like cells isolated from ICMs had a normal karyotype and highly expressed alkaline phosphatase. Multiple differentiation potency of the ES-like cells was confirmed by differentiation into neural cells and fibroblast-like cells in vitro. These results suggest that mouse ES cells might secrete factors playing important roles in promoting goat ES-like cells' self-renewal, moreover, the feeder layers and primary materials could also influence the successful isolation of goat ES-like cells.
山羊胚胎干细胞样细胞可从原始材料——内细胞团（ICM）中分离出来，并在含有小鼠胚胎干细胞条件培养基（ESCCM）的新培养体系中，于小鼠胚胎成纤维细胞（MEF）饲养层上保持未分化状态传代八次。然而，当在不含小鼠ESCCM的培养基中培养时，山羊胚胎干细胞样细胞传代三次以上便无法存活。此外，当ICM在山羊胚胎成纤维细胞上培养，或者原始材料——完整的山羊囊胚在MEF上培养时，均无法获得胚胎干细胞样细胞。从ICM分离出的山羊胚胎干细胞样细胞具有正常的核型，并高表达碱性磷酸酶。通过体外分化为神经细胞和成纤维细胞样细胞，证实了该胚胎干细胞样细胞具有多向分化潜能。这些结果表明，小鼠胚胎干细胞可能分泌了对促进山羊胚胎干细胞样细胞自我更新起重要作用的因子，此外，饲养层和原始材料也会影响山羊胚胎干细胞样细胞的成功分离。
