Zavala D C, Rhodes M L, Richerson H B, Oskvig R
J Allergy Clin Immunol. 1975 Dec;56(6):450-63. doi: 10.1016/0091-6749(75)90063-9.
A localized Arthus reaction was produced in the lung of sensitized rabbits by delivery of antigen into a lower lobe bronchus using a method of selective bronchial catheterization under fluoroscopy. The rabbits were sensitized with bovine immunoglobulin G (B-IgG) in incomplete Freund's adjuvant (IFA) to produce precipitating antibody without classic delayed hypersensitivity. Pulmonary histopathology was studied at intervals following antigen challenge, using light and immunofluorescent microscopy. Gross lesions peripheral to the lower lobe bronchus receiving antigen were found within 12 hr. Subsequent necrosis resulted in a dense scar by 6 wk. Microscopically, early lesions were typified by localized bronchitis, bronchiolitis, alveolitis, and vasculitis with exuberant exudates containing predominantly polymorphonuclear leukocytes. Extensive focal necrosis was present by 72 hr. Immunofluorescent studies revealed the presence of B-IgG, rabbit IgG, and complement (C3) in and around bronchi, bronchioles, alveoli, and vessels. No granulomatous lesions were found, and proliferation of alveolar lining cells was not detected in these studies. Thus, the lung can participate in an acute Arthus reaction following local antigen challenge in systemically sensitized animals. The pathology more closely resembles a necrotizing bacterial pneumonia than an interstitial or hypersensitivity pneumonitis under the conditions of this experimental system. Implications for human disease are speculative.
通过在荧光透视引导下采用选择性支气管插管法将抗原注入致敏兔的下叶支气管，在其肺部产生局部阿瑟斯反应。用牛免疫球蛋白G（B-IgG）加不完全弗氏佐剂（IFA）使兔致敏，以产生沉淀抗体而无典型的迟发型超敏反应。在抗原激发后的不同时间间隔，用光镜和免疫荧光显微镜研究肺组织病理学。在12小时内可发现接受抗原的下叶支气管周围有肉眼可见的病变。随后的坏死在6周时形成致密瘢痕。镜下，早期病变的特征为局部支气管炎、细支气管炎、肺泡炎和血管炎，伴有以多形核白细胞为主的大量渗出物。72小时时出现广泛的局灶性坏死。免疫荧光研究显示在支气管、细支气管、肺泡和血管内及周围存在B-IgG、兔IgG和补体（C3）。在这些研究中未发现肉芽肿性病变，也未检测到肺泡衬里细胞的增殖。因此，在全身致敏动物中，肺在局部抗原激发后可参与急性阿瑟斯反应。在此实验系统条件下，其病理表现更类似于坏死性细菌性肺炎，而非间质性或过敏性肺炎。对人类疾病的影响只是推测性的。
