聚乙二醇（PEG）衍生物及聚乙二醇化肽生物聚合物共轭物的合成。

Synthesis of polyethylene glycol (PEG) derivatives and PEGylated-peptide biopolymer conjugates.

作者信息

Li Jing, Kao W John

机构信息

Division of Pharmaceutical Sciences of the School of Pharmacy and Department of Biomedical Engineering of the College of Engineering, University of Wisconsin--Madison, Madison, Wisconsin 53705, USA.

出版信息

Biomacromolecules. 2003 Jul-Aug;4(4):1055-67. doi: 10.1021/bm034069l.

DOI:10.1021/bm034069l

PMID:12857092

Abstract

We synthesized a library of 50 poly(ethylene glycol) (PEG) derivatives to expand the extent of conjugation with biologically active molecules (biopolymers, peptides, drugs, etc.) and biomaterial substrates. The formation of PEG derivatives was confirmed with HPLC, (1)H and (13)C NMR. PEG derivatives were polymerized into networks in order to study the role of PEG and terminal functional groups in modulating the hydrophilicity of biomaterials and cell-biomaterial interaction. The resulting surface hydrophilicity and the number of adhered fibroblasts were primarily dependent on the PEG concentration with the molecular weight and the terminal functional group of PEG derivatives being less important. One of PEG derivatives, PEG-bis-glutarate, was utilized to link peptide sequences to gelatin backbone in the formation of novel biomedical hydrogels. PEG-peptide conjugates were characterized by mass spectroscopy. PEG-peptide modified gelatins were characterized by gel permeation chromatography.

摘要

我们合成了一个包含50种聚乙二醇（PEG）衍生物的文库，以扩大与生物活性分子（生物聚合物、肽、药物等）和生物材料底物的共轭程度。通过高效液相色谱、氢核磁共振（¹H NMR）和碳核磁共振（¹³C NMR）确认了PEG衍生物的形成。将PEG衍生物聚合成网络，以研究PEG和末端官能团在调节生物材料亲水性以及细胞与生物材料相互作用中的作用。所得的表面亲水性和成纤维细胞粘附数量主要取决于PEG浓度，而PEG衍生物的分子量和末端官能团的影响较小。其中一种PEG衍生物，聚乙二醇双谷氨酸酯，被用于在新型生物医学水凝胶的形成过程中将肽序列连接到明胶主链上。通过质谱对PEG-肽共轭物进行了表征。通过凝胶渗透色谱对PEG-肽修饰的明胶进行了表征。