School of Advanced Agricultural Science, Weifang University, Weifang 261061, China.
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China.
Molecules. 2024 Oct 19;29(20):4952. doi: 10.3390/molecules29204952.
A series of hybrid hydrogels of poly(ethylene glycol) (PEG) were synthesized using gelatin as a crosslinker and investigated for controlled delivery of the first-generation cephalosporin antibiotic, Cefazedone sodium (CFD). A commercially available 4-arm-PEG-OH was first modified to obtain four-arm-PEG-succinimidyl glutarate (4-arm-PEG-SG), which formed the gelatin-PEG composite hydrogels (SN) through crosslinking with gelatin. To regulate the drug delivery, SN hydrogels with various solid contents and crosslinking degrees were prepared. The effect of solid contents and crosslinking degrees on the thermal, mechanical, swelling, degradation, and drug release properties of the hydrogels were intensively investigated. The results revealed that increasing the crosslinking degree and solid content of SN could not only enhance the thermal stability, swelling ratio (SR), and compression resistance capacity of SN but also prolong the degradation and drug release times. The release kinetics of the SN hydrogels were found to follow the first-order model, suggesting that the transport rate of CFD within the matrix of hydrogels is proportional to the concentration of the drug where it is located. Specifically, SN-III showed 90% mass loss after 60 h of degradation and a sustained release duration of 72 h. The cytotoxicity assay using the MTT method revealed that cell viability rates of SN were higher than 95%, indicating excellent cytocompatibility. This study offers new insights and methodologies for the development of hydrogels as biomedical composite materials.
一系列聚乙二醇（PEG）的杂化水凝胶是通过明胶作为交联剂合成的，并研究了第一代头孢菌素抗生素头孢唑酮钠（CFD）的控制释放。首先，将一种市售的 4 臂-PEG-OH 进行修饰，得到 4 臂-PEG-琥珀酰亚胺戊二酸酯（4 臂-PEG-SG），通过与明胶交联形成明胶-PEG 复合水凝胶（SN）。为了调节药物释放，制备了具有不同固含量和交联度的 SN 水凝胶。详细研究了固含量和交联度对水凝胶的热性能、力学性能、溶胀、降解和药物释放性能的影响。结果表明，增加 SN 的交联度和固含量不仅可以提高 SN 的热稳定性、溶胀比（SR）和抗压能力，还可以延长降解和药物释放时间。SN 水凝胶的释放动力学遵循一级模型，表明 CFD 在水凝胶基质中的传输速率与其所在位置的药物浓度成正比。具体而言，SN-III 在 60 h 的降解后，质量损失达到 90%，并具有 72 h 的持续释放时间。采用 MTT 法进行的细胞毒性试验表明，SN 的细胞存活率高于 95%，表明其具有良好的细胞相容性。这项研究为水凝胶作为生物医学复合材料的发展提供了新的思路和方法。
