人类糖尿病性心耳中β1和β2肾上腺素能受体表达降低。
Decreased expression of beta1- and beta2-adrenoceptors in human diabetic atrial appendage.
作者信息
Dinçer U Deniz, Güner Sahika, Tay Aydin, Arioğlu Ebru, Taşdelen Atilay, Aşlamaci Sait, Bidasee Keshore R
机构信息
Department of Pharmacology, Faculty of Pharmacy, University of Ankara, Ankara, Turkey.
出版信息
Cardiovasc Diabetol. 2003 Jun 20;2:6. doi: 10.1186/1475-2840-2-6.
BACKGROUND
Using the streptozotocin-induced diabetic rat model, we have recently showed that the expression and function of beta1-adrenoreceptor were decreased in the diabetic rat heart. However, the effect of diabetes on expression of beta-adrenoreceptors in human cardiac tissue remains undefined. Therefore, the focus of the present study was to investigate the effect of diabetes on mRNA encoding beta1- and beta2-ARs in human atrial tissues.
METHODS
Right atrial appendages from five diabetic (mean age 65 +/- 4.5; 4 female, 1 male) and five nondiabetic patients (mean age 56.2 +/- 2.8; 4 male, 1 female) undergoing coronary artery bypass grafting were collected and assayed using reverse transcriptase-polymerase chain reaction (RT-PCR) for their mRNA content. No patient from these two groups suffered from acute myocardial infarction and/or failure. All diabetic patients received insulin for at least two years and had been diagnosed as diabetics for at least five years.
RESULTS
When compared with levels in nondiabetics, steady state levels of mRNA encoding beta1-adrenoreceptor decreased by 69.2 +/- 7.6% in diabetic patients while beta2-adrenoreceptor mRNA decreased by 32.2 +/- 5.5% (p < 0.001).
CONCLUSIONS
Our findings show a decreased expression of beta1- and beta2-adrenoreceptors in human diabetic atrial appendage.
背景
利用链脲佐菌素诱导的糖尿病大鼠模型，我们最近发现糖尿病大鼠心脏中β1-肾上腺素能受体的表达和功能降低。然而，糖尿病对人心脏组织中β-肾上腺素能受体表达的影响仍不明确。因此，本研究的重点是探讨糖尿病对人心房组织中编码β1-和β2-肾上腺素能受体（ARs）mRNA的影响。
方法
收集5例糖尿病患者（平均年龄65±4.5岁；4例女性，1例男性）和5例非糖尿病患者（平均年龄56.2±2.8岁；4例男性，1例女性）行冠状动脉搭桥术时的右心耳，采用逆转录聚合酶链反应（RT-PCR）检测其mRNA含量。这两组患者均无急性心肌梗死和/或心力衰竭。所有糖尿病患者接受胰岛素治疗至少两年，且被诊断为糖尿病至少五年。
结果
与非糖尿病患者相比，糖尿病患者中编码β1-肾上腺素能受体的mRNA稳态水平下降了69.2±7.6%，而β2-肾上腺素能受体mRNA下降了32.2±5.5%（p<0.001）。
结论
我们的研究结果表明，人糖尿病心房心耳中β1-和β2-肾上腺素能受体的表达降低。
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