Cui Lei, Li Fa-cheng, Zhang Qun, Qian Yun-liang, Guan Wen-xiang
Department of Plastic Surgery, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Chin J Traumatol. 2003 Aug;6(4):199-204.
To evaluate the effect of local application of vascular endothelial growth factor (VEGF) via adenovirus-mediated gene transfer on survival of full thickness flaps selected randomly in rats.
Thirty Sprague-Dawley rats weighing 480-520 g were used in this study. A dorsal flap (8 cm x 2 cm) in full thickness with the pedicle located at the level of the iliac crest was designed. Then the rats received 1,012 pfu replication-deficient recombinant adenovirus carrying VEGF (AdCMV-VEGF group, n=10), 1,012 pfu recombinant beta-galactosidase adenovirus (AdCMV-Gal group, n=10) and 1 ml saline (saline group, n=10), respectively, in the distal two thirds of the proposed flap by means of subdermal injection at 8 different locations. Three days after treatment, the flaps were elevated as originally designed and sutured back in situ. The survival rate of the flaps was evaluated on day 7 after operation.
The survival rate of the flaps in the AdCMV-VEGF group increased significantly as compared with those of the AdCMV-Gal group (P<0.01) and the saline group (P<0.01). Immunohistochemical staining showed that VEGF was expressed in the survival flaps injected with AdCMV-VEGF. Histological analysis showed that more granulation tissues and angiogenesis were observed in the AdCMV-VEGF group than those in the AdCMV-Gal and the saline groups.
Local application of adenovirus-mediated VEGF165 cDNA may efficiently improve the survival of ischemic skin flaps.
通过腺病毒介导的基因转移局部应用血管内皮生长因子（VEGF），评估其对随机选取的大鼠全层皮瓣存活的影响。
本研究选用30只体重480 - 520 g的Sprague-Dawley大鼠。设计一个蒂位于髂嵴水平的背部全层皮瓣（8 cm×2 cm）。然后，将大鼠分别在拟制备皮瓣远侧三分之二处的8个不同位置皮下注射1012 pfu的携带VEGF的复制缺陷型重组腺病毒（AdCMV-VEGF组，n = 10）、1012 pfu的重组β-半乳糖苷酶腺病毒（AdCMV-Gal组，n = 10）和1 ml生理盐水（生理盐水组，n = 10）。治疗3天后，按原设计掀起皮瓣并原位缝合。术后第7天评估皮瓣存活率。
与AdCMV-Gal组（P < 0.01）和生理盐水组（P < 0.01）相比，AdCMV-VEGF组皮瓣存活率显著提高。免疫组织化学染色显示，在注射AdCMV-VEGF的存活皮瓣中VEGF表达。组织学分析显示，AdCMV-VEGF组比AdCMV-Gal组和生理盐水组观察到更多的肉芽组织和血管生成。
腺病毒介导的VEGF165 cDNA局部应用可有效提高缺血皮瓣的存活率。
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