Malva João Oliveira, Vieira Ana P, Ambrósio António F, Oliveira Catarina R
Center for Neuroscience and Cell Biology of Coimbra, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, 3004-504 Coimbra, Portugal.
Neuroreport. 2003 May 6;14(6):847-50. doi: 10.1097/00001756-200305060-00014.
Activation of calcium permeable glutamate receptors is likely to be important for neuronal death associated with brain trauma, stroke and neurodegenerative diseases. Cobalt uptake can be used to identify cells containing Ca2+-permeable non-NMDA ionotropic glutamate receptors. However, the relative contribution of AMPA and kainate receptors, and also the role of receptor desensitization on the influx of Co2+, remain to be established. We found that the selective non-desensitizing activation of AMPA receptors was efficient in promoting Co2+ staining. However, the selective activation of kainate receptors, even under non-desensitizing conditions, did not result in Co2+ staining. Taken together, our results show that non-desensitizing stimulation of AMPA, but not of kainate receptors, mediates the influx of Co2+ in cultured rat hippocampal neurons.
钙通透性谷氨酸受体的激活可能对于与脑外伤、中风及神经退行性疾病相关的神经元死亡具有重要意义。钴摄取可用于识别含有Ca2+通透性非NMDA离子otropic谷氨酸受体的细胞。然而，AMPA和海人藻酸受体的相对贡献以及受体脱敏对Co2+内流的作用仍有待确定。我们发现，AMPA受体的选择性非脱敏激活在促进Co2+染色方面是有效的。然而，即使在非脱敏条件下，海人藻酸受体的选择性激活也不会导致Co2+染色。综上所述，我们的结果表明，AMPA而非海人藻酸受体的非脱敏刺激介导了培养的大鼠海马神经元中Co2+的内流。
