Capillary zone electrophoretic determination of iodide in seawater using transient isotachophoresis with artificial seawater as the background electrolyte.

We describe an application of capillary zone electrophoresis (CZE) with transient isotachophoresis (ITP) as the on-line concentration procedure for the determination of iodide in seawater. The effective mobility of iodide was decreased by the addition of 10 mM cetyltrimethylammonium chloride (CTAC) to an artificial seawater background electrolyte (BGE) so that transient ITP functioned and iodide was separated from other coexisting anions such as bromide, nitrite, and nitrate in seawater samples. After sample injection, 600 mM acetate was separately injected into the capillary as the terminating ion to generate transient ITP. The limit of detection (LOD) for iodide was 3.0 microg/L. The LOD was obtained at a signal-to-noise ratio (S/N) of 3. The values of the relative standard deviation (RSD) of peak area, peak height, and migration time for iodide were 2.9, 2.1, and 0.6%. The proposed method was applied to the determination of iodide in seawater collected around the Osaka Bay. The results obtained by use of the calibration graph were agreed with those obtained by the addition of the standard solutions for iodide.