Morel Jacques, Simoes Clarisse Da Silva, Avinens Odile, Sany Jacques, Combe Bernard, Eliaou Jean-Francois
Service d'Immunorhumatologie, Hôpital Lapeyronie, Montpellier, France.
J Rheumatol. 2003 Jul;30(7):1485-90.
To evaluate the contribution of HLA-DM alleles to susceptibility to systemic lupus erythematosus (SLE) in a Caucasian population.
HLA-DMA and DMB alleles were studied in 73 patients with SLE, 147 randomly selected controls, and 86 HLA-DRB1 genotype matched controls by oligotyping of polymerase chain reaction amplified genomic DNA with sequence-specific oligonucleotide probes.
There was a significant presence of HLA-DMA0103, DMA0104, and DMB0102 in the SLE patients compared with the randomly selected controls. After stratification of patients and matched controls according to DRB1 genotypes, only HLA-DMA0104 was increased in SLE patients negative for the SLE susceptibility HLA-DR alleles. For the patients and controls positive for HLA-DR allele-susceptibility for SLE, HLA-DMA0103, DMA0104, DMB0102, and DMB0103 alleles tended to be more frequent, but without reaching statistical significance. No correlation was found between HLA-DM phenotype frequencies and any clinical or biological manifestations of SLE.
This is the first study evaluating the influence of HLA-DM in a Caucasian SLE population. Our results suggest that HLA-DMA*0104 may represent a novel allele of susceptibility to SLE.
评估HLA - DM等位基因对白种人群系统性红斑狼疮（SLE）易感性的影响。
采用序列特异性寡核苷酸探针聚合酶链反应扩增基因组DNA的寡核苷酸分型方法，对73例SLE患者、147例随机选择的对照以及86例HLA - DRB1基因型匹配的对照进行HLA - DMA和DMB等位基因研究。
与随机选择的对照相比，SLE患者中HLA - DMA0103、DMA0104和DMB0102显著存在。根据DRB1基因型对患者和匹配对照进行分层后，仅在SLE易感性HLA - DR等位基因阴性的SLE患者中HLA - DMA0104增加。对于HLA - DR等位基因对SLE易感的患者和对照，HLA - DMA0103、DMA0104、DMB0102和DMB0103等位基因倾向于更频繁出现，但未达到统计学意义。未发现HLA - DM表型频率与SLE的任何临床或生物学表现之间存在相关性。
这是第一项评估HLA - DM对白种人SLE人群影响的研究。我们的结果表明，HLA - DMA*0104可能代表一种新的SLE易感等位基因。
