Pulis Randy P, Wu Beatrice M, Zhu Jay Z, Castillo Erika G, Kneteman Norman M, Churchill Thomas A
Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada.
Am J Transplant. 2003 Aug;3(8):961-7. doi: 10.1034/j.1600-6143.2003.00166.x.
The purpose of this study was to clarify the metabolic events that explain why supplemental glucose is detrimental during cardiac storage. Four solutions were used to flush and store porcine hearts: St. Thomas Hospital Solution (STHS), University of Wisconsin (UW) solution, and UW + 90 mM histidine, and UW + 90 mM histidine + 11 mM glucose. Despite equivalent increases in lactate in the two histidine-buffered groups throughout 10 h of storage, glycogen utilization was evident in the group without supplemental glucose. The presence of glucose resulted in a reduction in energy production, presumably mediated by direct inhibition of glycogenolysis. Furthermore, UW + histidine was the only group to show consistent improvements in ATP and total adenylates. It was concluded that inclusion of the buffering agent, histidine, to UW solution promotes anaerobic energy production as a result, in part, of preserved high levels of the regulatory control enzyme, phosphofructokinase.
本研究的目的是阐明那些能解释为何补充葡萄糖在心脏保存过程中有害的代谢事件。使用四种溶液对猪心脏进行冲洗和保存：圣托马斯医院溶液（STHS）、威斯康星大学（UW）溶液、UW + 90 mM组氨酸溶液以及UW + 90 mM组氨酸 + 11 mM葡萄糖溶液。在整个10小时的保存过程中，尽管两个组氨酸缓冲组的乳酸增加量相当，但在未补充葡萄糖的组中糖原利用明显。葡萄糖的存在导致能量产生减少，推测是由对糖原分解的直接抑制介导的。此外，UW +组氨酸是唯一显示ATP和总腺苷酸持续改善的组。得出的结论是，在UW溶液中加入缓冲剂组氨酸可促进无氧能量产生，部分原因是保留了高水平的调节控制酶磷酸果糖激酶。
