从巴西热带土壤中分离出的链霉菌新菌株培养滤液中的蛋白质合成抑制活性。
Protein synthesis inhibitory activity in culture filtrates from new strains of Streptomyces isolated from Brazilian tropical soils.
作者信息
Reis S A, Costa L V, Cavalcanti E D C, Giambiagi-deMarval M, Semêdo L T A S, Coelho R R R, Moussatché N, Damaso C R A
机构信息
Laboratório de Biologia Molecular de Vírus, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
出版信息
Lett Appl Microbiol. 2003;37(2):138-43. doi: 10.1046/j.1472-765x.2003.01363.x.
AIMS
To investigate the effect of the culture supernatants from three newly isolated Streptomyces strains, 221, 235 and 606 on eukaryotic cells.
METHODS AND RESULTS
Cell lines were treated with the culture filtrates and assayed for protein synthesis by metabolic labelling, followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis. RNA synthesis was investigated by [5-3H]uridine incorporation. The three culture filtrates presented a strong inhibitory activity, reducing total protein synthesis of different eukaryotic cell lines by more than 85%. No effect on cellular RNA synthesis was detected. The culture filtrates did not affect the growth of the prokaryotic cells tested.
CONCLUSIONS
These new Streptomyces strains, recently isolated from Brazilian tropical soils, produce molecule(s) with inhibitory activity specific to eukaryote protein synthesis.
SIGNIFICANCE AND IMPACT OF THE STUDY
Streptomyces strains 221, 235 and 606, probably representing new species, might produce new bioactive compound(s), and can be used as valuable tools to study the protein synthesis pathway in eukaryotes.
目的
研究新分离的三株链霉菌菌株221、235和606的培养上清液对真核细胞的影响。
方法与结果
用培养滤液处理细胞系，通过代谢标记法检测蛋白质合成，随后进行十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析。通过[5 - ³H]尿苷掺入法研究RNA合成。三种培养滤液呈现出较强的抑制活性，使不同真核细胞系的总蛋白质合成减少超过85%。未检测到对细胞RNA合成的影响。培养滤液不影响所测试原核细胞的生长。
结论
这些最近从巴西热带土壤中分离出的新链霉菌菌株产生了对真核生物蛋白质合成具有特异性抑制活性的分子。
研究的意义和影响
链霉菌菌株221、235和606可能代表新物种，可能产生新的生物活性化合物，可作为研究真核生物蛋白质合成途径的有价值工具。
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