Ma Yan-ping, Zou Ping, Xiao Juan, Huang Shi-ang
Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhonghua Xue Ye Xue Za Zhi. 2003 May;24(5):238-40.
To investigate the expression and significance of survivin protein and mRNA in cord blood (CB) CD(34)(+) stem/progenitor cells.
Survivin level in CB CD(34)(+) cells was assessed by immuno-histochemistry and reverse transcription polymerase chain reaction (RT-PCR).
Survivin expressed in fresh CB CD(34)(+) cells, its protein level was 9.4 +/- 1.2, faint signal of the mRNA transcript band was detected. In the in vitro culture of the cells, hematopoietic growth factors could upregulate survivin expression, especially the combination of SCF, FL and Tpo. After 3 days culture, its protein level was 25.2 +/- 5.3, and mRNA transcript increased significantly. Survivin expression dropped down 24 hours after withdraw of these cytokines.
Survivin is not a specific anti-apoptotic protein of cancer, and also expressed in normal hematopoietic stem/progenitor cells. It plays important regulating roles in the process of hematopoiesis.
探讨存活素蛋白及mRNA在脐血CD(34)(+)干/祖细胞中的表达及意义。
采用免疫组织化学和逆转录聚合酶链反应（RT-PCR）检测脐血CD(34)(+)细胞中存活素水平。
存活素在新鲜脐血CD(34)(+)细胞中表达，其蛋白水平为9.4±1.2，检测到mRNA转录带的微弱信号。在细胞体外培养中，造血生长因子可上调存活素表达，尤其是干细胞因子（SCF）、促血小板生成素（Tpo）和Flt3配体（FL）联合使用时。培养3天后，其蛋白水平为25.2±5.3，mRNA转录显著增加。撤除这些细胞因子24小时后，存活素表达下降。
存活素并非肿瘤特异性抗凋亡蛋白，在正常造血干/祖细胞中也有表达，在造血过程中发挥重要调节作用。
