Beck D P, Greenawalt J W
J Gen Microbiol. 1976 Jan;92(1):111-9. doi: 10.1099/00221287-92-1-111.
Changes in the capacity of mitochondria to carry out oxidative phosphorylation and in the rate of synthesis and incorporation of phospholipids into mitochondria were measured during the germination of conidiospores of Neurospora crassa. The competence of isolated mitochondria to carry out coupled respiration was very low during the first 3 h growth, but it increased rapidly, reaching maximal levels at 5 to 6 h growth. Changes in mitochondrial function were the same in cells grown in 2% sucrose- or 15% glucose-supplemented medium. The rate of synthesis of mitochondrial phospholipids was very low during the first 2 h growth and increased to maximal levels between 3 and 5 h. The rate of synthesis of mitochondrial phospholipids was approximately three times higher in cells grown in 15% glucose than in those grown in 2% sucrose. The maximal rate of synthesis of mitochondrial phospholipids occurred during spore germination and preceded attainment of full competence for oxidative phosphorylation. The lipid-rich condition of the mitochondrial resulting from the high rate of synthesis of phospholipids in glucose-grown cells is postulated to be related to the whorled inclusions observed in thin sections of Neurospora cells.
在粗糙脉孢菌分生孢子萌发过程中，测定了线粒体进行氧化磷酸化的能力以及磷脂合成和掺入线粒体的速率变化。在生长的最初3小时内，分离的线粒体进行偶联呼吸的能力非常低，但随后迅速增加，在生长5至6小时时达到最高水平。在添加2%蔗糖或15%葡萄糖的培养基中生长的细胞，线粒体功能的变化是相同的。线粒体磷脂的合成速率在生长的最初2小时非常低，并在3至5小时之间增加到最高水平。在15%葡萄糖中生长的细胞中，线粒体磷脂的合成速率大约是在2%蔗糖中生长的细胞的三倍。线粒体磷脂合成的最大速率发生在孢子萌发期间，并先于氧化磷酸化完全能力的获得。据推测，在葡萄糖生长的细胞中，由于磷脂合成速率高而导致的线粒体富含脂质的状态与在粗糙脉孢菌细胞薄片中观察到的轮状内含物有关。
