Smith E W, Slayman C W
J Bacteriol. 1977 Feb;129(2):836-42. doi: 10.1128/jb.129.2.836-842.1977.
Mitochondria from Neurospora crassa, like mammalian mitochondria, carry out rapid, energy-linked K+ uptake and H+ release in the presence of valinomycin. The maximal rate of K+ uptake was about 1.0 mumol/mg of mitochondrial protein per min and was seen at valinomycin concentrations in the range of 100 to 200 mug per mg of mitochondrial protein and at K+ concentrations of 4 mM or above. Uptake could be supported either by substrate oxidation or by adenosine 5'-triphosphate (ATP), and was inhibited in the former case by antimycin or cyanide, in the latter case by oligomycin, and in both cases by 2,4-dinitrophenol. Mitochondria from the cytochrome-deficient mutant poky carried out substrate-driven K+ uptake at reduced rates, but oligomycin-sensitive, ATP-driven K+ uptake at rates about 60% greater than those shown by wild-type mitochondria. This result is consistent with the recent finding (Mainzer and Slayman 1976) that poky contains elevated amounts of oligomycin-sensitive mitochondrial adenosine 5'-triphosphatase activity.
粗糙脉孢菌的线粒体与哺乳动物的线粒体一样，在缬氨霉素存在的情况下能快速进行与能量相关的钾离子摄取和氢离子释放。钾离子摄取的最大速率约为每分钟每毫克线粒体蛋白1.0微摩尔，在缬氨霉素浓度为每毫克线粒体蛋白100至200微克且钾离子浓度为4毫摩尔或更高时可见。摄取可由底物氧化或三磷酸腺苷（ATP）支持，在前一种情况下被抗霉素或氰化物抑制，在后一种情况下被寡霉素抑制，在两种情况下均被2,4 -二硝基苯酚抑制。来自细胞色素缺陷突变体迟缓型的线粒体以较低速率进行底物驱动的钾离子摄取，但对寡霉素敏感的、由ATP驱动的钾离子摄取速率比野生型线粒体高约60%。这一结果与最近的发现（Mainzer和Slayman，1976年）一致，即迟缓型含有升高量的对寡霉素敏感的线粒体三磷酸腺苷酶活性。
