Zhang Zhihua, Burns Darcy C, Kumita Janet R, Smart Oliver S, Woolley G Andrew
Department of Chemistry, University of Toronto, 80 St. George St., Toronto M5S 3H6, Canada.
Bioconjug Chem. 2003 Jul-Aug;14(4):824-9. doi: 10.1021/bc0340161.
We have designed and synthesized a water-soluble, sulfonated version of an azobenzene-based thiol-reactive cross-linker that can be introduced into peptides and proteins and act as a conformational photoswitch. The sulfonated compound is shown to effect a similar degree of conformational control on a model peptide helix system, as its nonsulfonated counterpart but can be introduced without the need for any organic cosolvent. The sulfonated azobenzene cross-linker thus expands the range of proteins to which photocontrol can be applied.
我们设计并合成了一种基于偶氮苯的硫醇反应性交联剂的水溶性磺化版本，它可以引入到肽和蛋白质中并作为一种构象光开关。结果表明，这种磺化化合物对模型肽螺旋系统的构象控制程度与未磺化的对应物相似，但引入时无需任何有机助溶剂。因此，磺化偶氮苯交联剂扩大了可应用光控的蛋白质范围。
