Hook Sarah, Camberis Mali, Prout Melanie, Le Gros Graham
School of Pharmacy, University of Otago, P.O. Box 913, Dunedin, New Zealand.
J Neuroimmunol. 2003 Jul;140(1-2):61-8. doi: 10.1016/s0165-5728(03)00168-1.
Certain forms of the neuroendocrine hormone preproenkephalin (PPNK) are produced by T cells, B cells and macrophages. This hormone has been shown to be important in regulating a variety of immune responses; however, the basic mechanisms of this regulation are unknown. Here we examine the ability of CD8 and CD4 PPNK-deficient T lymphocytes to proliferate to antigenic and mitogenic stimuli. We found that lymphocyte activation and proliferation to suboptimal concentrations of both anti-CD3 and antigen was reduced in the absence of PPNK. Proliferation could be rescued by increasing antigen or by co-incubation of PPNK-deficient cells with wild-type cells. These data confirm the importance of neuroendocrine hormones such as PPNK in T cell activation and proliferation and provides a potential mechanism for the regulation of T cell responses by PPNK or its peptide derivatives.
神经内分泌激素前脑啡肽原（PPNK）的某些形式由T细胞、B细胞和巨噬细胞产生。这种激素已被证明在调节多种免疫反应中很重要；然而，这种调节的基本机制尚不清楚。在这里，我们研究了CD8和CD4 PPNK缺陷型T淋巴细胞对抗原性和有丝分裂刺激的增殖能力。我们发现，在缺乏PPNK的情况下，淋巴细胞对亚最佳浓度的抗CD3和抗原的激活和增殖减少。通过增加抗原或使PPNK缺陷型细胞与野生型细胞共同孵育，可以挽救增殖。这些数据证实了诸如PPNK等神经内分泌激素在T细胞激活和增殖中的重要性，并为PPNK或其肽衍生物调节T细胞反应提供了一种潜在机制。
