Shen Wei-zai, Lin Hong-sheng, Guo Guo-qing
Department of Anatomy, Medical College of Jinan University, Guangzhou 510630, China.
Di Yi Jun Yi Da Xue Xue Bao. 2003 Jul;23(7):692-5.
To explore the role of nitric oxide in the development of diabetes mellitus by observing the changes of neuronal nitric oxide synthase (nNOS)-immunoreactive (nNOS-ir) neurons in the paraventricular hypothalamic nucleus (PVN) of diabetic rats.
Diabetic rat models were established by means of intraperitoneal streptozotocin injections. At the end of 2, 7 and 12 weeks after model establishment, tissue sampling was performed and the number of nNOS-ir neurons in PVN were counted and quantitatively analyzed.
The number of nNOS-ir neurons in PVN of diabetic rats remained normal 2 weeks after model establishment (P >0.05) but was significantly increased by the time of 7 weeks (P <0.05); at the 12th weeks, the number of nNOS-ir neurons of diabetic group was still greater than that of the control group (P <0.05).
The number of nNOS-ir neurons in PVN increases significantly in middle and advanced stages of diabetes, suggesting the possible relations between the nNOS activity changes in PVN and the changes in cerebral neuroendocrine and adrenal activity in diabetic condition.
通过观察糖尿病大鼠下丘脑室旁核（PVN）中神经元型一氧化氮合酶（nNOS）免疫反应阳性（nNOS-ir）神经元的变化，探讨一氧化氮在糖尿病发生发展中的作用。
采用腹腔注射链脲佐菌素建立糖尿病大鼠模型。在模型建立后2、7和12周结束时进行组织取样，对PVN中nNOS-ir神经元的数量进行计数并进行定量分析。
糖尿病大鼠模型建立后2周，PVN中nNOS-ir神经元数量保持正常（P>0.05），但在7周时显著增加（P<0.05）；在第12周时，糖尿病组nNOS-ir神经元数量仍高于对照组（P<0.05）。
糖尿病中晚期PVN中nNOS-ir神经元数量显著增加，提示PVN中nNOS活性变化与糖尿病状态下脑神经内分泌及肾上腺活动变化之间可能存在关联。
