Tomita T, Doull V
Department of Pathology, University of Kansas Medical Center, Kansas City 66103.
Diabetes Res. 1992 Feb;19(2):49-58.
Morphometric analysis of pancreatic islets was performed on BB/W rats, which were divided into moderately diabetic and severely diabetic as well as normoglycemic during four months of observation. In normoglycemic rats, total islet area occupied 1.2% of total pancreatic tissue area with a mean area of 19,000 microns2 per islet. Total pancreatic area was markedly decreased to 22% and 10% in moderately and severely diabetic rats, respectively, compared with that of normoglycemic rats. Mean islet area of moderately and severely diabetic rats decreased to 28% and 25% per islet that of normoglycemic rats, respectively, in which severely diabetic rats showed variable sizes of islets. Concomitant morphometric analysis of renal glomeruli showed decreasing sizes of glomeruli in severely diabetic rats whereas number of glomeruli in renal cortex increased in severely diabetic rats compared with normoglycemic rats. Moderately and severely diabetic states were also studied by I.V. glucose and insulin tolerance tests in regard to plasma glucose and insulin levels compared with normoglycemic rats. During months of observation severely diabetic rats had their total islet area reduced to 10% that of normoglycemic rats as a result of cell mediated immune destruction of islets. And concomitant reduction of glomeruli may be a part of diabetic glomerulopathy. Yet, diabetic rats were normotensive. The degree of water intake, urine volume, urine glucose, urine protein and albumin was also correlated with the degree of diabetes. This study further provides the feasibility of using diabetic BB/W rats for diabetic conditions and their complications.
对BB/W大鼠进行胰岛形态计量学分析，在四个月的观察期内，将其分为中度糖尿病组、重度糖尿病组以及血糖正常组。在血糖正常的大鼠中，胰岛总面积占胰腺组织总面积的1.2%，每个胰岛的平均面积为19000平方微米。与血糖正常的大鼠相比，中度和重度糖尿病大鼠的胰腺总面积分别显著减少至22%和10%。中度和重度糖尿病大鼠的胰岛平均面积分别降至血糖正常大鼠的28%和25%，其中重度糖尿病大鼠的胰岛大小不一。对肾小球进行的形态计量学分析显示，与血糖正常的大鼠相比，重度糖尿病大鼠的肾小球尺寸减小，而肾皮质中的肾小球数量增加。还通过静脉注射葡萄糖和胰岛素耐量试验研究了中度和重度糖尿病状态下与血糖正常大鼠相比的血浆葡萄糖和胰岛素水平。在观察期内，由于胰岛的细胞介导免疫破坏，重度糖尿病大鼠的胰岛总面积减少至血糖正常大鼠的10%。肾小球的相应减少可能是糖尿病肾小球病变的一部分。然而，糖尿病大鼠血压正常。水摄入量、尿量、尿糖、尿蛋白和白蛋白的程度也与糖尿病程度相关。本研究进一步提供了将糖尿病BB/W大鼠用于糖尿病病情及其并发症研究的可行性。
