Kuebler Joachim F, Toth Balazs, Rue Loring W, Wang Ping, Bland Kirby I, Chaudry Irshad H
Center for Surgical Research, University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USA.
Shock. 2003 Aug;20(2):144-8. doi: 10.1097/01.shk.0000072127.33223.f1.
Gender differences in immune and organ functions have been described in different rodent models of trauma- and pressure-controlled hemorrhagic shock. We hypothesized that gender influences the regulation of plasma and tissue fluids in rats under such conditions. To study this we used male and weight matched proestrus female Sprague-Dawley rats, which were assigned to three groups (n = 7/group): sham, maximal bleedout (trauma and 45 min of blood pressure at 35 mmHg without resuscitation), or 5 h after completion of trauma-hemorrhage and resuscitation. Trauma-hemorrhage involved midline laparotomy and approx. 90 min of hemorrhagic shock (35 mmHg), followed by fluid resuscitation (4x the shed blood volume with Ringers lactate). (51)Cr-EDTA, (125)I-albumin distribution, and wet weight/dry weight were used to calculate plasma volume and extracellular fluid volume and cellular water content. Proestrus female rats showed significantly higher plasma volumes compared with weight-matched males. The volume of blood withdrawn in the first 15 min of hemorrhagic shock was significantly less in proestrus females compared with males; however, there was no significant difference in the total shed blood volume. Moreover, proestrus females showed less interstitial edema formation compared with male rats at 5 h after resuscitation. We conclude that differences in the regulation of plasma and tissue volumes exist between males and proestrus females during and after trauma-hemorrhage. The increased circulating blood volume could contribute the improved immune and organ functions in proestrus females under those conditions.
在创伤和压力控制的失血性休克的不同啮齿动物模型中，已经描述了免疫和器官功能的性别差异。我们假设，在这种情况下，性别会影响大鼠血浆和组织液的调节。为了研究这一点，我们使用了雄性和体重匹配的动情前期雌性斯普拉格-道利大鼠，将它们分为三组（每组n = 7）：假手术组、最大放血组（创伤并在35 mmHg血压下持续45分钟且不进行复苏），或创伤性出血和复苏完成后5小时。创伤性出血包括中线剖腹术和约90分钟的失血性休克（35 mmHg），随后进行液体复苏（用乳酸林格氏液补充失血体积的4倍）。使用（51）铬-乙二胺四乙酸、（125）碘-白蛋白分布以及湿重/干重来计算血浆容量、细胞外液容量和细胞含水量。与体重匹配的雄性大鼠相比，动情前期雌性大鼠的血浆容量显著更高。与雄性大鼠相比，动情前期雌性大鼠在失血性休克的前15分钟内抽出的血量显著更少；然而，总失血量没有显著差异。此外，在复苏后5小时，与雄性大鼠相比，动情前期雌性大鼠的间质水肿形成较少。我们得出结论，在创伤性出血期间和之后，雄性和动情前期雌性大鼠在血浆和组织容量调节方面存在差异。在这些情况下，循环血量增加可能有助于改善动情前期雌性大鼠的免疫和器官功能。
