Tsai Tsuimin, Chen Hsin-Ming, Wang Chih-Yu, Tsai Jui-Chang, Chen Chin-Tin, Chiang Chun-Pin
Graduate Institute of Biomedical Materials, Taipei Medical University, Taipei, Taiwan.
Lasers Surg Med. 2003;33(1):40-7. doi: 10.1002/lsm.10180.
To test whether autofluorescence spectroscopy can be used for the diagnosis of oral neoplasia in a high-risk population, we characterized the in vivo autofluorescence spectra from oral submucous fibrosis (OSF) lesions and oral premalignant and malignant lesions in both OSF and non-OSF patients.
STUDY DESIGN/MATERIALS AND METHODS: Autofluorescence emission spectra were measured under the excitation wavelength of 330 nm, using a Xenon lamp-based fluorospectrometer coupled to a handheld optical fiber probe. Autofluorescence spectroscopies were analyzed among patients with OSF lesions, and oral lesions of epithelial hyperkeratosis (EH), epithelial dysplasia (ED), and squamous cell carcinomas (SCC) and normal oral mucosa (NOM) of healthy volunteers.
We found that the most intensely autofluorescence emission peaks occurred at 380 nm and 460 nm. For comparing the spectral patterns among different groups of oral lesions and NOM, ratios of the area under the spectrum of 460+/-10 nm to that under the spectrum of 380+/-10 nm (denoted as A(460+/-10nm)/A(380+/-10nm)) were calculated. The mean ratio values increased gradually from OSF to NOM, to EH and ED, and to SCC. The ANOVA test showed significant differences in the ratio value among all categories of samples (P<0.01). On the other hand, we found that EH, ED, and SCC lesions on OSF patients had distorted autofluorescence intensity. The mean ratio values of EH, ED, and SCC between non-OSF and OSF patients show significant differences. Furthermore, an ANOVA test showed NOM is not distinguishable from EH and ED lesions on oral fibrotic mucosa (P>0.05).
Autofluorescence spectroscopy can be used to diagnose EH, ED, and SCC lesions in non-OSF patients but not in OSF patients.
为了测试自体荧光光谱是否可用于高危人群口腔肿瘤的诊断，我们对口腔黏膜下纤维化（OSF）患者以及OSF和非OSF患者的口腔癌前病变及恶性病变的体内自体荧光光谱进行了特征分析。
研究设计/材料与方法：使用与手持式光纤探头相连的基于氙灯的荧光光谱仪，在330nm激发波长下测量自体荧光发射光谱。对患有OSF病变、上皮过度角化（EH）、上皮发育异常（ED）和鳞状细胞癌（SCC）的患者以及健康志愿者的正常口腔黏膜（NOM）进行自体荧光光谱分析。
我们发现最强的自体荧光发射峰出现在380nm和460nm处。为了比较不同组口腔病变和NOM之间的光谱模式，计算了460±10nm光谱下的面积与380±10nm光谱下的面积之比（记为A(460±10nm)/A(380±10nm)）。平均比值从OSF到NOM、再到EH和ED、最后到SCC逐渐升高。方差分析显示所有类别样本的比值存在显著差异（P<0.01）。另一方面，我们发现OSF患者的EH、ED和SCC病变的自体荧光强度发生了畸变。非OSF和OSF患者中EH、ED和SCC的平均比值显示出显著差异。此外，方差分析显示NOM与口腔纤维化黏膜上的EH和ED病变无法区分（P>0.05）。
自体荧光光谱可用于诊断非OSF患者的EH、ED和SCC病变，但不能用于诊断OSF患者的此类病变。
