Olucha-Bordonau Francisco E, Teruel Vicent, Barcia-González Jorge, Ruiz-Torner Amparo, Valverde-Navarro Alfonso A, Martínez-Soriano Francisco
Department of Anatomy and Human Embryology, Faculty of Medicine and Odontology, University of Valencia, E-46010 Valencia, Spain.
J Comp Neurol. 2003 Sep 8;464(1):62-97. doi: 10.1002/cne.10774.
The nucleus incertus is located caudal to the dorsal raphe and medial to the dorsal tegmentum. It is composed of a pars compacta and a pars dissipata and contains acetylcholinesterase, glutamic acid decarboxylase, and cholecystokinin-positive somata. In the present study, anterograde tracer injections in the nucleus incertus resulted in terminal-like labeling in the perirhinal cortex and the dorsal endopyriform nucleus, the hippocampus, the medial septum diagonal band complex, lateral and triangular septum medial amygdala, the intralaminar thalamic nuclei, and the lateral habenula. The hypothalamus contained dense plexuses of fibers in the medial forebrain bundle that spread in nearly all nuclei. Labeling in the suprachiasmatic nucleus filled specifically the ventral half. In the midbrain, labeled fibers were observed in the interpeduncular nuclei, ventral tegmental area, periaqueductal gray, superior colliculus, pericentral inferior colliculus, pretectal area, the raphe nuclei, and the nucleus reticularis pontis oralis. Retrograde tracer injections were made in areas reached by anterogradely labeled fibers including the medial prefrontal cortex, hippocampus, amygdala, habenula, nucleus reuniens, superior colliculus, periaqueductal gray, and interpeduncular nuclei. All these injections gave rise to retrograde labeling in the nucleus incertus but not in the dorsal tegmental nucleus. These data led us to conclude that there is a system of ascending projections arising from the nucleus incertus to the median raphe, mammillary complex, hypothalamus, lateral habenula, nucleus reuniens, amygdala, entorhinal cortex, medial septum, and hippocampus. Many of the targets of the nucleus incertus were involved in arousal mechanisms including the synchronization and desynchronization of the theta rhythm.
不确定核位于中缝背核的尾侧和被盖背侧的内侧。它由致密部和弥散部组成，包含乙酰胆碱酯酶、谷氨酸脱羧酶和胆囊收缩素阳性的躯体。在本研究中，向不确定核注射顺行示踪剂导致在嗅周皮质、背侧梨状叶内核、海马、内侧隔斜角带复合体、外侧隔和三角隔、内侧杏仁核、丘脑板内核和外侧缰核出现终末样标记。下丘脑在内侧前脑束中有密集的纤维丛，几乎分布于所有核团。视交叉上核中的标记物特别填充了腹侧半。在中脑，在脚间核、腹侧被盖区、导水管周围灰质、上丘、中央下丘周、顶盖前区、中缝核和脑桥嘴侧网状核中观察到标记纤维。向顺行标记纤维所到达的区域注射逆行示踪剂，这些区域包括内侧前额叶皮质、海马、杏仁核、缰核、连合核、上丘、导水管周围灰质和脚间核。所有这些注射均在不确定核中产生逆行标记，但在被盖背核中未产生。这些数据使我们得出结论，存在一个从不确定核向上投射到中缝正中核、乳头体复合体、下丘脑、外侧缰核、连合核、杏仁核、内嗅皮质、内侧隔和海马的系统。不确定核的许多靶区都参与觉醒机制，包括θ节律的同步化和去同步化。
