Komuro Y, Watanabe T, Hosoi Y, Matsumoto Y, Nakagawa K, Saito S, Ishihara S, Kazama S, Tsuno N, Kitayama J, Suzuki N, Tsurita G, Muto T, Nagawa H
Dept. of Surgical Oncology, Faculty of Medicine, University of Tokyo, Japan.
J Exp Clin Cancer Res. 2003 Jun;22(2):223-8.
The identification of predictive indicators of radiosensitivity is extremely useful in selecting patients suited for preoperative radiotherapy and avoiding unnecessary preoperative treatment. In this study, we evaluated the possible role of the immunohistochemical expression pattern of p53 and Ku70 protein in determining tumor radiosensitivity in rectal cancer before preoperative irradiation. We examined pretreatment biopsy materials from 111 patients by immunohistochemistry. The expression pattern of p53 and Ku70 was evaluated for association with tumor radiosensitivity, which was defined according to the criteria of the Japanese Research Society for Cancer of the Colon and Rectum. There was a significant correlation between the expression pattern of p53 and tumor radiosensitivity (P = 0.045); Ku70 and tumor radiosensitivity (P < 0.001); and the combination of p53 and Ku70, and tumor radiosensitivity (P < 0.001). The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy in both p53 and Ku70-positive cases for radioresistance were all superior to those of the group positive for p53 alone. In conclusion the examination of the combination of p53 and Ku70 may predict the radiosensitivity of rectal cancer before preoperative irradiation.
识别放射敏感性的预测指标对于选择适合术前放疗的患者以及避免不必要的术前治疗极为有用。在本研究中，我们评估了p53和Ku70蛋白的免疫组化表达模式在术前放疗前确定直肠癌肿瘤放射敏感性方面的可能作用。我们通过免疫组化检查了111例患者的治疗前活检材料。根据日本结肠直肠癌研究学会的标准，评估p53和Ku70的表达模式与肿瘤放射敏感性之间的关联。p53的表达模式与肿瘤放射敏感性之间存在显著相关性（P = 0.045）；Ku70与肿瘤放射敏感性之间存在显著相关性（P < 0.001）；p53和Ku70的组合与肿瘤放射敏感性之间存在显著相关性（P < 0.001）。p53和Ku70均阳性病例对放射抵抗的敏感性、特异性、阳性预测值、阴性预测值和准确性均优于单独p53阳性组。总之，p53和Ku70联合检测可能预测术前放疗前直肠癌的放射敏感性。
