Nunnari J J, Fisher M, White S D
Department of Pathology, University of Massachusetts Medical School, Worcester 01655.
Exp Mol Pathol. 1992 Dec;57(3):193-204. doi: 10.1016/0014-4800(92)90010-9.
We studied the effects of clentiazem, a calcium channel blocker (1) on the accumulation of lipid in the aorta, (2) on the level of plasma lipids, and (3) on the number of adherent intimal monocytes and foam cells. Seventy Wistar rats were assigned to one of the following groups: (1) regular diet, (2) an atherogenic diet consisting of regular chow with 2% cholesterol, 1% cholic acid, and 0.5% thiouracil (CCT), (3) CCT supplemented with 5 mg/kg/day clentiazem, and (4) CCT with 25 mg/kg/day clentiazem. Animals were sacrificed after 6 or 12 weeks of diet. Aortas were studied by light microscopy after staining with oil red O (ORO) and/or hematoxylin. ORO staining was quantified in both abdominal and thoracic regions of the aorta. The aortas of the clentiazem groups demonstrated significantly less ORO staining than CCT diet controls in thoracic aorta after 6 weeks and abdominal aorta after 12 weeks. There was no significant difference in the plasma lipid concentrations. The clentiazem-treated groups had fewer numbers of adherent monocytes and foam cells. We conclude that clentiazem inhibits lipid deposition in cholesterol-fed rats without lowering plasma lipid concentrations and that the number of intimal monocytes and foam cells is decreased in the presence of this calcium antagonist.
（1）主动脉中脂质的蓄积；（2）血浆脂质水平；（3）内膜单核细胞和泡沫细胞的数量。70只Wistar大鼠被分为以下几组：（1）常规饮食组；（2）致动脉粥样硬化饮食组，饮食由含2%胆固醇、1%胆酸和0.5%硫脲嘧啶的常规饲料组成（CCT）；（3）补充5mg/kg/天克仑硫卓的CCT组；（4）补充25mg/kg/天克仑硫卓的CCT组。在饮食6周或12周后处死动物。用苏丹红O（ORO）和/或苏木精染色后，通过光学显微镜对主动脉进行研究。对主动脉的腹部和胸部区域的ORO染色进行定量分析。克仑硫卓组的主动脉在6周后胸部主动脉以及12周后腹部主动脉的ORO染色明显少于CCT饮食对照组。血浆脂质浓度无显著差异。克仑硫卓治疗组的黏附单核细胞和泡沫细胞数量较少。我们得出结论，克仑硫卓在不降低血浆脂质浓度的情况下抑制胆固醇喂养大鼠的脂质沉积，并且在这种钙拮抗剂存在的情况下内膜单核细胞和泡沫细胞的数量会减少。
