Liang Guo, Li Defa, Wang Fenglai, Dai Jianguo, Yang Wenjun
TianJin Agricultural College, TianJin, China.
Arch Tierernahr. 2003 Apr;57(2):117-25. doi: 10.1080/0003942031000107316.
Twelve crossbred barrows (BW 47 +/- 2.81 kg) were fitted with a T-cannula in the terminal ileum and fed one of ten diets containing corn gluten meal, dried distillers grain or dried distillers grain with solubles as the sole protein source, according to a 6 x 6 Latin Square design. Lysine, tryptophan and methionine were, the first, second and third limiting amino acids in these by-products. The ileal apparent digestibility of methionine, tyrosine, leucine and phenylalanine in corn gluten meal was high. The ileal apparent digestibility of tryptophan, tyrosine and leucine differed significantly in corn gluten meal. The ileal apparent digestibility of amino acids was higher in dried distillers grain with solubles than in dried distillers grain. To predict the ileal apparent digestibility of tryptophan, methionine and threonine linear regression equations were derived. The results suggested that processing technique can influence the ileal apparent digestibility ofamino acids in corn by-products. It was concluded that the direct technique cannot accurately determine the ileal apparent digestibility of tryptophan in dried distillers grain with solubles and dried distillers grain, due to its low content. The content of an amino acid in a feedstuff used as the sole source of protein must meet the minimum requirement of pigs when the ileal apparent digestibility of constituent amino acids is determined using the direct technique.
十二头杂交公猪(体重47±2.81千克)在回肠末端安装了T型套管,并按照6×6拉丁方设计,饲喂十种日粮中的一种,这些日粮分别以玉米蛋白粉、干酒糟或带可溶物的干酒糟作为唯一蛋白质来源。赖氨酸、色氨酸和蛋氨酸分别是这些副产品中的第一、第二和第三限制性氨基酸。玉米蛋白粉中蛋氨酸、酪氨酸、亮氨酸和苯丙氨酸的回肠表观消化率较高。玉米蛋白粉中色氨酸、酪氨酸和亮氨酸的回肠表观消化率差异显著。带可溶物的干酒糟中氨基酸的回肠表观消化率高于干酒糟。为预测色氨酸、蛋氨酸和苏氨酸的回肠表观消化率,推导了线性回归方程。结果表明,加工工艺会影响玉米副产品中氨基酸的回肠表观消化率。得出的结论是,由于色氨酸含量低,直接法不能准确测定带可溶物的干酒糟和干酒糟中色氨酸的回肠表观消化率。当使用直接法测定组成氨基酸的回肠表观消化率时,用作唯一蛋白质来源的饲料中氨基酸的含量必须满足猪的最低需求。