离子液体1-丁基-3-甲基咪唑六氟磷酸盐中扩散的荧光相关光谱研究。

Fluorescence correlation spectroscopic studies of diffusion within the ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate.

作者信息

Werner James H, Baker Sheila N, Baker Gary A

机构信息

Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

出版信息

Analyst. 2003 Jun;128(6):786-9. doi: 10.1039/b300734k.

DOI:10.1039/b300734k

PMID:12866904

Abstract

Fluorescence correlation spectroscopy (FCS) measurements have been performed in order to determine the translational diffusion coefficients of three differently charged fluorescent probes (cationic: rhodamine 6G, neutral: 4-dicyanomethylene-2-methyl-6-p-dimethylaminostyryl-4-H-pyran, DCM, and anionic: fluorescein) dissolved within the common room temperature ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate, [bmim][PF6]. These experiments demonstrate that FCS is a rapid, sensitive, precise (typical RSD from 4-8%), and low-consumption screening tool for the determination of analyte mobility within microliter ionic liquid samples.

摘要

为了测定溶解在常见室温离子液体1-丁基-3-甲基咪唑六氟磷酸盐（[bmim][PF6]）中的三种带不同电荷的荧光探针（阳离子型：罗丹明6G、中性型：4-二氰基亚甲基-2-甲基-6-对二甲氨基苯乙烯基-4-H-吡喃，DCM，以及阴离子型：荧光素）的平移扩散系数，进行了荧光相关光谱（FCS）测量。这些实验表明，FCS是一种快速、灵敏、精确（典型相对标准偏差为4 - 8%）且低消耗的筛选工具，用于测定微升离子液体样品中分析物的迁移率。