Lee Jia-Lin, Chang Ching-Jin, Chueh Ling-Ling, Lin Chung-Tien
Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei 106, Taiwan, Republic of China.
In Vitro Cell Dev Biol Anim. 2003 May-Jun;39(5-6):221-7. doi: 10.1290/1543-706X(2003)039<0221:EOSFPI>2.0.CO;2.
Canine mammary tumors (CMTs) have been proposed to be a good animal model for human breast cancer. To provide a basis for the tumorigenic study of CMTs, cell lines were established using a modified cell culture technique. The epithelial morphology and immunostaining with cytokeratin 18 confirmed the epithelial origin of the cells. In an investigation of possible mammary tumorigenesis-related factors, the expression of Wnt signaling-related proteins was detected in cell lines. Secreted frizzled-related protein 2 (SFRP2) was abundantly expressed in CMT cells but not in normal canine mammary gland (MG) cells. Secreted frizzled-related protein 2 was secreted into the culture medium and was associated with the extracellular matrix. In addition, increased expressions of beta-catenin and cyclin D1 were observed in cells overexpressing SFRP2. The marked differential expression of SFRP2 reveals that this protein may be a potential candidate marker for CMTs. The CMT cell line established in this study provides a useful tool and experimental model for understanding both the tumorigenesis of CMTs and the role of Wnt signaling in cancers.
犬乳腺肿瘤（CMTs）已被认为是人类乳腺癌的良好动物模型。为了为CMTs的致瘤性研究提供依据，采用改良的细胞培养技术建立了细胞系。上皮形态和细胞角蛋白18免疫染色证实了细胞的上皮来源。在对可能与乳腺肿瘤发生相关的因素进行的一项研究中，在细胞系中检测了Wnt信号相关蛋白的表达。分泌型卷曲相关蛋白2（SFRP2）在CMT细胞中大量表达，但在正常犬乳腺（MG）细胞中不表达。分泌型卷曲相关蛋白2分泌到培养基中，并与细胞外基质相关。此外，在过表达SFRP2的细胞中观察到β-连环蛋白和细胞周期蛋白D1的表达增加。SFRP2的显著差异表达表明该蛋白可能是CMTs的潜在候选标志物。本研究建立的CMT细胞系为理解CMTs的肿瘤发生以及Wnt信号在癌症中的作用提供了有用的工具和实验模型。
