Francioni E, Fillmann G, Hamacher C, Wagener A De Luca R, Depledge M H, Readman J W, Meniconi M De Fátima Guadalupe
Pontifícia Universidade Católica do Rio de Janeiro, Depto. de Química, Marques de São Vicemte, 225, Gávea, Rio de Janeiro, RJ, Brazil, 22453-900.
Environ Technol. 2003 Jun;24(6):665-70. doi: 10.1080/09593330309385601.
The reliability of enzyme-linked immunosorbent assay (ELISA) tests as a screening technique to address groundwater contamination was tested in an area following leakage of gasoline from a petrol station. Immunoassay data of benzene, toluene, ethylbenzene, and o-, m- and p-xylene (BTEX) were compared with results obtained using capillary gas chromatographic analysis. Detection limits were of 20 microg l(-1) for ELISA and 0.3 microg l(-1) for gas chromatography with flame ionization and photoionization detectors (GC-FID/PID) determination. Despite an observed overestimation of BTEX concentrations as given by ELISA, the tests responded reliably to different levels of contamination.
在一个加油站发生汽油泄漏后的区域，对酶联免疫吸附测定（ELISA）测试作为一种用于检测地下水污染的筛选技术的可靠性进行了测试。将苯、甲苯、乙苯以及邻、间、对二甲苯（BTEX）的免疫测定数据与使用毛细管气相色谱分析获得的结果进行了比较。ELISA的检测限为20微克/升，而使用火焰离子化和光离子化检测器（GC-FID/PID）的气相色谱法测定的检测限为0.3微克/升。尽管ELISA给出的BTEX浓度存在高估现象，但该测试对不同程度的污染做出了可靠反应。
