Oehme Albrecht, Gaschler Gisela, Straube Eberhard
Institute of Medical Microbiology, Friedrich-Schiller-University Jena, Jena, Germany.
Int J Med Microbiol. 2003 Jun;293(2-3):225-8. doi: 10.1078/1438-4221-00238.
Urogenital strains of Chlamydia trachomatis are divided into several serogroups (D-K). Since these serovars are represented with differing prevalence in the population a serotyping of strains is necessary, when characterising the epidemiological situation. The aim of this study was the genotyping of C. trachomatis strains, the comparison of the results with those of serotyping, and the genotyping of positive specimens using commercial nucleic acid amplification tests (NAAT). The Chlamydia trachomatis major outer membrane protein gene (omp1) from 55 isolated strains and 36 NAAT-positive specimens was amplified by polymerase chain reaction (PCR). The restriction fragment length polymorphism (RFLP) patterns of these amplicons were compared with those of reference strains. The genotypes E and F were found to be most prevalent. The results are discussed considering other studies, genovariants and epidemiology.
沙眼衣原体泌尿生殖系统菌株分为几个血清群（D-K）。由于这些血清型在人群中的流行率不同，因此在描述流行病学情况时，有必要对菌株进行血清分型。本研究的目的是对沙眼衣原体菌株进行基因分型，将结果与血清分型结果进行比较，并使用商业核酸扩增试验（NAAT）对阳性标本进行基因分型。通过聚合酶链反应（PCR）扩增了55株分离菌株和36份NAAT阳性标本中的沙眼衣原体主要外膜蛋白基因（omp1）。将这些扩增产物的限制性片段长度多态性（RFLP）模式与参考菌株的模式进行比较。发现基因型E和F最为常见。结合其他研究、基因变体和流行病学对结果进行了讨论。
