Morré S A, Ossewaarde J M, Lan J, van Doornum G J, Walboomers J M, MacLaren D M, Meijer C J, van den Brule A J
Department of Pathology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands.
J Clin Microbiol. 1998 Feb;36(2):345-51. doi: 10.1128/JCM.36.2.345-351.1998.
Urogenital isolates (n = 93) of Chlamydia trachomatis were differentiated into serovars and variants by serotyping with monoclonal antibodies and genotyping by restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified omp1 gene, respectively. The types of 87 of the 93 isolates (94%) were identical, as determined by both methods. Among these 87 isolates, 3 isolates were identified as the recently described new serovariant Ga/IOL-238 by omp1 nucleotide sequence analysis of the variable domains. Of the remaining six isolates, three isolates serotyped as both L2 and Ba but were identified as Ba/A-7 by genotyping by RFLP analysis of omp1. The omp1 nucleotide sequences of variable domains VD1, VD2, and VD4 of these urogenital Ba strains were identical to the sequences of the variable domains of Ba/J160, an ocular Ba type. The three remaining isolates were serotyped as J, but the patterns obtained by RFLP analysis of omp1, which were identical for the three isolates, differed from that of prototype serovar J/UW36. omp1 nucleotide sequence analysis revealed that these strains are genovariants of serovar J/UW36. Nucleotide sequence differences between serovar J/UW36 and this J genovariant, designated Jv, were found in both variable and constant domains. In conclusion, this study shows that the PCR-based genotyping of clinical C. trachomatis isolates by RFLP analysis of omp1 has a higher discriminatory power and is more convenient than serotyping. Variants of C. trachomatis serovars Ba, G, and J were identified and characterized.
沙眼衣原体泌尿生殖道分离株（n = 93）分别通过用单克隆抗体进行血清分型和对PCR扩增的omp1基因进行限制性片段长度多态性（RFLP）分析进行基因分型，从而区分血清型和变异型。两种方法确定的93株分离株中的87株（94%）类型相同。在这87株分离株中，通过对可变区进行omp1核苷酸序列分析，有3株被鉴定为最近描述的新血清变异型Ga/IOL - 238。其余6株分离株中，3株血清分型为L2和Ba，但通过对omp1进行RFLP分析的基因分型被鉴定为Ba/A - 7。这些泌尿生殖道Ba菌株可变区VD1、VD2和VD4的omp1核苷酸序列与眼部Ba型Ba/J160可变区的序列相同。其余3株分离株血清分型为J，但通过对omp1进行RFLP分析得到的图谱，这3株分离株相同，与原型血清型J/UW36不同。omp1核苷酸序列分析表明这些菌株是血清型J/UW36的基因变异型。在血清型J/UW36和这个被命名为Jv的J基因变异型之间，在可变区和恒定区均发现了核苷酸序列差异。总之，本研究表明，通过对omp1进行RFLP分析对临床沙眼衣原体分离株进行基于PCR的基因分型具有更高的鉴别力，并且比血清分型更方便。鉴定并表征了沙眼衣原体血清型Ba、G和J的变异型。
