Ibrahim M A, Ellis J S, Chain B M, Katz D R
Department of Histopathology, University College and Middlesex School of Medicine, London, England.
Immunobiology. 1992 Nov;186(5):362-77. doi: 10.1016/s0171-2985(11)80391-8.
The migratory behavior of antigen-presenting cells was investigated in vivo. Purified murine splenic dendritic cells and splenic and peritoneal macrophages were labelled and injected subcutaneously in the hind foot-pads of mice and monitored for seven days. In the first 24 h, a small quantity of label was recovered from popliteal but not inguinal lymph nodes with radioactive (111In-oxine and 3H-uridine) but not fluorescent (1,1'-dioctadecyl 3,3,3'3'-tetramethylindocarbocyanine perchlorate and fluorescein isothiocyanate) labelling of the antigen-presenting cells. Chemical fixation of the injected antigen-presenting cells had no effect on the detection of label in the popliteal lymph nodes, suggesting that it was unlikely to be due to active cellular migration. Label recovery from hind feet declined with time over the seven day period and was independent of the label type. Essentially the same observations were made whether the antigen-presenting cells were syngeneic or allogeneic to the injected mice and irrespective of the type of antigen-presenting cell used. However, allogeneic antigen-presenting cells, which did not migrate to the draining lymph nodes, successfully primed T lymphocytes in these lymph nodes as shown by a secondary in vitro mixed leukocyte reaction. Again, chemical fixation of the injected antigen-presenting cells had no effect on their ability to prime allogeneic T lymphocytes in the draining lymph nodes. These experiments suggest that, during experimental allo-sensitization via the subcutaneous route, indirect priming of allogeneic T lymphocytes may be a dominant pathway.
在体内研究了抗原呈递细胞的迁移行为。纯化的小鼠脾脏树突状细胞以及脾脏和腹膜巨噬细胞被标记后，皮下注射到小鼠的后足垫中，并监测七天。在最初的24小时内，用放射性标记（111铟-奥克辛和3H-尿苷）而非荧光标记（1,1'-二辛基3,3,3'3'-四甲基吲哚羰花青高氯酸盐和异硫氰酸荧光素）标记抗原呈递细胞后，从腘窝淋巴结而非腹股沟淋巴结中回收了少量标记物。注射的抗原呈递细胞的化学固定对腘窝淋巴结中标记物的检测没有影响，这表明这不太可能是由于活跃的细胞迁移所致。在七天的时间里，后足的标记物回收率随时间下降，且与标记类型无关。无论抗原呈递细胞与注射小鼠是同基因还是异基因，也无论使用何种类型的抗原呈递细胞，基本都得到了相同的观察结果。然而，未迁移至引流淋巴结的异基因抗原呈递细胞，如通过二次体外混合淋巴细胞反应所示，成功地在这些淋巴结中启动了T淋巴细胞。同样，注射的抗原呈递细胞的化学固定对其在引流淋巴结中启动异基因T淋巴细胞的能力没有影响。这些实验表明，在通过皮下途径进行实验性同种致敏过程中，异基因T淋巴细胞的间接启动可能是主要途径。
